EzWay™ Transfection Reagent

• Cationic Lipid 성분의 Transfection Reagent입니다.
• Plasmid DNA와 RNAi vector의 cell로의 transfection이 용이합니다.
• Stems cell에서도 transfection이 용이합니다.
• Serum의 유무에 관계없이 transfection efficiency가 높습니다.
• 세포 독성이 없어 중간에 media를 교체 해주지 않아도 되며, 세포 viability가 매우 높습니다.
• in vitro와 in vivo 모두에 보편적으로 사용할 수 있습니다.
• Lipoplex (liposome & DNA mixture)를 만들기가 쉽습니다.
• Enhancer와 혼합할 필요가 없는 one-step 용액 입니다.
• 24-well plate 기준으로 250 transfection을 할 수 있는 충분한 양이 제공됩니다.
• Patent No. : 2003-0075611

Cell Line List

Cell Line	Cell Type
HeLa	Human cervix adenocarcinoma
293A	Human kidney cancer
B16BL6	Mouse skin melanoma
B16F10	Mouse skin melanoma
MCF-7	Human breast adenocarcinoma
HepG2	Human liver/hepatocellular carcinoma
Hep-3B	Human hepatocellular carcinoma
Chang	Human normal liver cell
BT-20	Human breast carcinoma
TC-1	Mouse lung tumor
CT-26	Mouse colon carcinoma
EMT-6	Mouse breast cancer
SK-OV-3	Human ovarian adenocarcinoma
A549	Human lung epithelial carcinoma
MDA-MB-231	Human breast adenocarcinoma
NCI	Human lung cancer (carcinoma)
SK-Br-3	Human breast carcinoma
HT-29	Human colorectal adenocarcinoma
Jurkat	Human T-cell leukemia

• Plasmid Transfection
• RNAi or siRNA Transfection
• Stem Cell Transfection

실험과정

사용예
• Efficient delivery of DNA to a wide variety of cell lines
  (A)
  (B)

  Using the EzWay™ Transfection Reagent or competitor reagent L, cells were transfected with the pDNA, which expresses luciferase (A) and GFP (B) respectively. One microgram of pDNA complexed to EzWay ™(1:4.9 wt ratios of pDNA and lipid) was added to a various cell lines, The cells were transfected for 4 hours and incubated for additional 24 hours. The luciferase activity (A) of the cell lysate was measured with a luminometer and a luciferase assay kit and the GFP expression (B) was determined by fluorescence microscopy.
• Knockdown efficacy of EzWayTM Transfection Reagent
  

  siRNA targeting luciferase was transfected by EzWay™ Transfection Reagent and competitor reagent L into Hep-3B cells. For B16BL6 cells, siRNA targeting luciferase was co-transfected with luciferase gene by two products, respectively. Luciferase-silencing efficiency was calculated relative to untreated cells. (A) shows the luciferase expression, while (B) shows the reduction in luciferase activity. EzWay™ Transfection Reagent delivered significant gene silencing from 10 nM of renilla luciferase siRNA.
• Inhibition of GFP expression in cells transfected with GFP targeting siRNA using EzWayTM Transfection Reagent
  

  MDA-MB-231 cells constitutively expressing the GFP gene were transfected with 10 nM final siRNA concentration of GFP-targeting siRNA.

• siIMPORTER Transfection Reagent