MilliTrace™ Constitutive GFP Reporter Adult Rat Hippocampal NSC Kit

• 안정한 GFP가 이식된 세포는 FACS에서 95~100%의 purity를 나타냅니다.
• Constitutive GFP Reporter Adult Rat Hippocampal NSCs는 Nestin+Sox2+TuJ1-GFAP-이 NSC를 immunochemical staining하는 특성을 보여주며, 이러한 NSC cell들은 다기능을 보여줍니다.
• Defined neuronal differentiation conditions (Rodent Neuron Differentiation Kit, Cat. No. SCR035)상태에서 cell들의 대부분은 βIII-tubulin positive neurons (GFP+βIII-tubulin+GFAP-)으로 분화됩니다.
• Defined astrocytic differentiation conditions (Astrocyte Differentiation Medium, Cat. No. SCM010)상태에서 cell들의 대부분은 GFAP-positive astrocytes (GFP+GFAP+βIII-tubulin-)로 분화됩니다.

• 제품 구성
  • MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells, 1 x 10⁶ viable cells
  • MilliTrace Rat Neural Stem Cell Expansion Medium (Cat. No. SCM040)

• Characterization of Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCC080)
  

  Figure 1. Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCC080) have a normal karyotype (A) and constitutively express GFP (B, D, G) along with NSC markers, Nestin (C, E) and Sox-2 (F, H). Nuclei of the cells were visualized with DAPI (blue). The Sox-2 transcription factor is co-localized with the GFP staining in the nucleus. Majority of cells are GFP-positive (B, D, G).

  

  Figure 2. Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem
  Cells are multipotent. Using Rodent Neuron Differentiation Kit (Catalog No. SCR035), NSC can differentiate into neurons (βIII-tubulin, A, C, red). Using Rodent Astrocyte Differentiation Medium (Catalog No. SCM010) NSC can differentiate into astrocytes (GFAP, D, F, red). Majority of cells are GFPpositive(B, E).

• ENStem-A™ Human Neural Progenitor
• Embryonic Stem Cells (ESC)
• Feeder Cells
• Mesenchymal Stem Cells (MSC)
• Neural Stem Cells (NSC)
• ReNcell Human Neural Progenitors
• Rodent Cell Lines