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 제품안내  Assay Kit   Cell-Based Assay Kit  Stem Cell Assay

Bone Marrow Harvesting and HSC Isolation Kit

골내막과 골수 중심부로부터 Hematopoietic stem cells (HSC)과 Bone marrow cell (BMC)을 얻기위한 제품입니다.
Merck Millipore
제품 문의하기
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    특징
  • Hematopoietic stem cells (HSC)과 Bone marrow cell (BMC)을 얻을수 있도록 시약과 간단한 도구가 제공됩니다.
  • 뼈를 갈을수 있는 Mortar와 pestle와, 효소등이 제공됩니다.
  • 기존의 flushing method보다 6배 더 높은 primitive HSCs (Lineage- Sca-1+ and c-Kit+)를 회수할 수 있습니다.
    사양
  • 제품 구성
    • Mortar and pestle - 1 porcelain mortar and pestle
    • Cell Strainer - 20 cell strainers with 40 μm nylon mesh and blue polypropylene frame
    • Dispase II - 10 single use vials of 40 mg powdered enzyme
    • Collagenase I - 10 single use vials of 30 mg powdered enzyme
    적용
  • HSCs within the endosteal region have significantly increased homing efficiency to the bone marrow in vivo
    Characterization of Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCC080)

    Figure 1. HSC isolated from the endosteal region and transplanted into nonablated recipients had a significantly higher homing efficiency to the bone marrow after 15 hrs compared to HSC isolated from the central marrow core (p< 0.05) (A).
    In addition, HSC isolated from the endosteal region and transplanted into non-ablated recipients had a significantly higher affinity to relodge within the endosteal region of the bone marrow after 15 hrs compared to HSC isolated from the central marrow core (p < 0.05) (B). Data are the mean±SEM from both endosteal and central marrow HSC isolated from 15 animals and transplanted into 3 individual recipients.

  • HSCs within the endosteal region have significantly increased proliferative potential in vivo
    Characterization of Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCC080)

    Figure 2. Sorted HSC (100 per well) isolated from the endosteal region (black bar) and the central marrow core (white bar) were grown in serum free media in the presence of 4 stimulatory growth factors. After 6 days there was a significant (p=0.02, students t-test) increase in the number of cells generated from endosteal HSC compared to those isolated from the central marrow core. Data are from a representative experiment (n=4) showing the mean±SEM from quadruplicate wells.