Cardiac Stem Cell Isolation Kit

Cardiac stem cells (CSCs)은 심근 또는 혈관세포계열로 분화할수 있는 세포로, 심장 재생 연구 분야에 있어 이상적인 모델로 사용되고 있습니다.

하지만, CSC이 complex와 짝을 이루고 있어 분리하기가 쉽지 않은데, 본 키트를 이용하여 간단하게 세포를 분리해낼 수 있습니다.

Merck Millipore

제품 문의하기

인쇄하기

easy-to-use cell isolation kit이기 때문에 편리합니다.
더 이상 복잡한 프로토콜로 인한 시간낭비와 값비싼 cell sorting 장비가 필요 없습니다.
높은 yield와 순도가 높은 CSC의 개체를 얻을 수 있어 결과가 좋습니다.

제품 구성
- Cardiac Stem Cell Isolation Buffer
- Cardiac Tissue Dissociation Buffer
- Steriflip® 100 μm Nylon Net Filtration Devices (5 units)
- Gradient Solution 1
- Gradient Solution 2
- Cardiac Stem Cell Maintenance Medium
- Cardiomyocyte Differentiation Medium

Figure 1. Discrete cell populations can be isolated from ventricular heart tissue through differential gradient centrifugation. Representative photos depicting heterogeneous cell populations present in the lower phase before centrifugation and a pure CSC population present in the upper phase after centrifugation.

Figure 2. Cardiac stem cells can be cultured in vitro and differentiated through selected use of growth or differentiation media. Representative images of low (A) and high (B) density cultures of purified CSCs and 12-day differentiated cardiomyocytes (C).

Figure 3. Cultured CSCs retain their stem cell characteristics and efficiently differentiate into cardiomyocytes. One week cultures of purified CSCs ubiquitously express stem cell markers, Sca-1 (A) and telomerase (B), while remaining in a proliferative state as determined by Ki67 (C) immunoreactivity. Differentiated CSCs express mature markers for cardiomyocytes, troponin I (D), desmin (E) and actinin (F), using the Cardiomyocyte Characterization Kit (Catalog No. SCR059). Cell nuclei were stained with DAPI (blue).

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