Embryonic Stem (ES) Cell Embryoid Body Formation Medium

• embryoid body (EB) 형성을 최적화하는 조건으로 제공되는 media 입니다.
• mouse ES cells을 neural, adipogenic, cardiomyogenic cell lineages로 분화시키거나, human ES cell의 분화를 유도하는데 이용합니다.

Figure 1. Representative images of EBs formed by the culture of murine embryonic stem cells (Catalog No. SCR012) in Embryoid Body Formation Medium for (A) two days and (B) four days.



Figure 2. Using the Murine ES Cell Neurogenesis Kit (Catalog. No. SCR101), mouse embryonic stem cells can be differentiated into (A) βIII-tubulin positive neurons (red). Cell nuclei were counterstained with DAPI (blue) (40 X magnification). (B) Using the Murine ES Cell Adipogensis Kit (Catalog. No. SCR100), mouse embryonic stem cells were differentiated into adipocytes as indicated by the accumulation of lipid vacuoles that stain with Oil Red O (10 X magnification).



Figure 3: To obtain beating cardiomyocytes, murine ES cells (Catalog No. SCR012) dissociated by Accutase were cultured in hanging drops (1000 cells/drop, 20 μL/drop) in Embryoid Body Formation Medium (Catalog No. SCM018) to induce embryoid body (EB) formation. After 5 days in a hanging drop culture, the resulting EBs were transferred to a 0.1% gelatin coated 96-well plate at a density of 1 EB/well in fresh Embryoid Body Formation Medium. Individual wells containing beating EBs were identified by eye, trypsinized, pooled and transferred to gelatin coated glass chamber slides in fresh Embryoid Body Formation Medium. After 48 hours of incubation, the slides were fixed with 4% PFA and stained with the indicated cardiomyocyte antibodies (Catalog No. SCR059).

• HEScGRO™ Medium for Human ES cells
• LIF (Leukemia Inhibitory Factor) and ESGRO
• Media for ESC Human, Mouse
• Media for NSC, MSC, HSC
• Mouse Embryonic Stem Cell Culture