Mouse Embryonic Stem Cell Adipogenesis Kit
|
|
|
|
|
Mouse Embryonic Stem Cell Adipogenesis Kit는 mouse ES cell을 지방세포(adipocytes)로 분화시키기 위한 reagent를 제공합니다. |
|
| Merck Millipore |
- mouse ES cell을 adipocyte로 분화시키기위해 필요한 reagent를 제공합니다.
- 분화된 adipocyte의 양을 측정하기위한 Oil Red O stain, wash, dye extraction solution등이 같이 제공됩니다.
- 제품 구성
- Embryoid Body (EB) Formation Medium - 500 mL
- Accutase Cell Dissociation Solution - 100 mL
- Inducer A Solution - 200 uL
- Inducer A Solution - 200 uL
- 3,3′,5-Triiodo-L-thyronine (T3) Solution - 200 uL
- 0.1% Gelatin Solution - 100 mL
- Oil Red O Solution - 60 mL
- Wash Solution - 250 mL
- Dye Extraction Solution - 30 mL
Figure 1. Undifferentiated mouse ES cells (Catalogue No. SCR012) display the characteristic tight round colonies with a high nuclear to cytoplasmic ratio, (A, 40 X magnification) and stain positively for mouse ES cell marker SSEA-1 (B, 40 X magnification).
Figure 2. Formation of embryoid bodies (EB) after the culture of dissociated mouse ES cells in EB Formation Medium for 2 days (A). Morphology of EBs after treatment with Inducer A Solution for 3 days (B). 10 X magnification.
Figure 3. 26-day differentiation of mouse ES cells to Oil Red O positive adipocytes (A) bright-field image, 20X magnification. (B, C) Oil Red O stained images, 10 and 20 X magnification, respectively).
Figure 4. Differentiation of mouse ES cells via T3 and insulin (induced) produced approximately twice the amount of oil red O stained adipocytes as compared to spontaneous differentiation (uninduced).
Blank represents the background staining of the plate (no cells included) due to absorbance of plates to the oil red O stain.
제품안내 
Assay Kit 
Webmaster for more information.
