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 Á¦Ç°¾È³»  Assay Kit   Cell-Based Assay Kit  Stem Cell Assay

Mouse Embryonic Stem Cell Neurogenesis Kit

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Embryonic stem cellµéÀº ¿Ü¹è¿±°ú Á߹迱 ±×¸®°í ³»¹è¿±À¸·Î ºÐÈ­µÉ ¼ö ÀÖ°í, ÀÌ·¯ÇÑ ¼¼Æ÷µéÀÇ ¼ºÀå Æ¯¼ºÈ­ ¶§¹®¿¡ neurogenesis¿Í in vitro¿Í in vivo»ó¿¡¼­ ´º·±ÀÇ ±â´É Àå¾Ö ¹ß»ý¿¡ ´ëÇÑ ¸ðµ¨·Î »ç¿ëµÇ°í ÀÖ½À´Ï´Ù.

Mouse Embryonic Stem Cell Neurogenesis Kit´Â mouse ES ¼¼Æ÷°¡ in vitro¿¡¼­ ¥âIII-tubulin positive neuronsÀ¸·Î ºÐÈ­µÇµµ·Ï ÃÖÀûÈ­ µÇ¾î ÀÖ½À´Ï´Ù.

Merck Millipore
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  • mouse ES cellÀ» neuronsÀ¸·Î ºÐÈ­½Ã۱âÀ§ÇØ ÇÊ¿äÇÑ reagent¸¦ Á¦°øÇÕ´Ï´Ù.
  • 4-/4+/8- ºÐÈ­ÇÁ·ÎÅäÄÝÀ» ÅëÇÏ¿©, ÃÑ 16ÀϾȿ¡ in vitro¿¡¼­ ¥âIII-tubulin positive neuronsÀ¸·Î ºÐÈ­µË´Ï´Ù.
  • * 4-(Embryoid Body formation)/4+(Neuronal Differentiation Induction)/8-(Expansion of Neuronal Cells from Embryoid Bodies)
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    • Embryoid Body (EB) Formation Medium- 500 mL
    • Accutase Cell Dissociation Soluton - 100 mL
    • Neural Inducer A Solution - 200 uL
    • Mouse Laminin - 200 ¥ìg
    • Poly-L-ornithine Solution - 2 mg
    • Mouse anti-SSEA-1 - 50 ¥ìg
    • Mouse anti-¥âIII tubulin - 50 ¥ìl
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    Figure 1. Undifferentiated mouse ES cells (Catalogue No. SCR012) display the characteristic tight round colonies with a high nuclear to cytoplasmic ratio, (A, 40 X magnification) and stain positively for mouse ES cell marker SSEA-1 (B, 40 X magnification).
    Figure 2. Formation of embryoid bodies (EB) after the culture of dissociated mouse ES cells in EB Formation Medium for 2 days (A) and 4 days (B) on a non-adhesive Petri dish. 10 X magnification.
    Figure 3. 26-day differentiation of mouse ES cells to Oil Red O positive adipocytes (A) bright-field image, 20X magnification. (B, C) Oil Red O stained images, 10 and 20 X magnification, respectively).
    Figure 4. Differentiation of mouse ES cells to ¥âIII-tubulin positive neurons (B, 20 X magnification; C, 40 X magnification). Bright-field image (A, 40 X magnification).
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
SCR101Mouse Embryonic Stem Cell Neurogenesis Kit10 reactions/Kit