Mouse Embryonic Stem Cell Neurogenesis Kit
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Mouse Embryonic Stem Cell Neurogenesis Kit´Â mouse ES ¼¼Æ÷°¡ in vitro¿¡¼ ¥âIII-tubulin positive neuronsÀ¸·Î ºÐȵǵµ·Ï ÃÖÀûÈ µÇ¾î ÀÖ½À´Ï´Ù. |
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| Merck Millipore |
- mouse ES cellÀ» neuronsÀ¸·Î ºÐȽÃ۱âÀ§ÇØ ÇÊ¿äÇÑ reagent¸¦ Á¦°øÇÕ´Ï´Ù.
- 4-/4+/8- ºÐÈÇÁ·ÎÅäÄÝÀ» ÅëÇÏ¿©, ÃÑ 16ÀϾȿ¡ in vitro¿¡¼ ¥âIII-tubulin positive neuronsÀ¸·Î ºÐȵ˴ϴÙ.
- * 4-(Embryoid Body formation)/4+(Neuronal Differentiation Induction)/8-(Expansion of Neuronal Cells from Embryoid Bodies)
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- Embryoid Body (EB) Formation Medium- 500 mL
- Accutase Cell Dissociation Soluton - 100 mL
- Neural Inducer A Solution - 200 uL
- Mouse Laminin - 200 ¥ìg
- Poly-L-ornithine Solution - 2 mg
- Mouse anti-SSEA-1 - 50 ¥ìg
- Mouse anti-¥âIII tubulin - 50 ¥ìl
Figure 1. Undifferentiated mouse ES cells (Catalogue No. SCR012) display the characteristic tight round colonies with a high nuclear to cytoplasmic ratio, (A, 40 X magnification) and stain positively for mouse ES cell marker SSEA-1 (B, 40 X magnification).
Figure 2. Formation of embryoid bodies (EB) after the culture of dissociated mouse ES cells in EB Formation Medium for 2 days (A) and 4 days (B) on a non-adhesive Petri dish. 10 X magnification.
Figure 3. 26-day differentiation of mouse ES cells to Oil Red O positive adipocytes (A) bright-field image, 20X magnification. (B, C) Oil Red O stained images, 10 and 20 X magnification, respectively).
Figure 4. Differentiation of mouse ES cells to ¥âIII-tubulin positive neurons (B, 20 X magnification; C, 40 X magnification). Bright-field image (A, 40 X magnification).