EzWay™ pink-ONE cytokine ELISA Kit

• Serum을 포함하여, CSF, plasma등 각종 biological 샘플에서의 cytokine을 측정할수 있습니다.
• Human, Mouse Rat 샘플에서의 다양한 cytokine을 정량할수 있습니다.
• 핑크빛을 띠는 Assay Diluent에 희석함으로서, 파이펫팅시 시약의 로딩위치를 알수 있습니다.
• 핑크빛을 띠는 pink-ONE TMB는 기존의 무색 TMB에 비해 로딩시 색상으로 구분이 되므로 파이펫팅 실수를 줄여줍니다.
• pink-ONE TMB는 이미 substrate와 buffer가 혼합되어 있어, 사용전 mixing 할 필요가 없어 간편합니다.
• 96well 샘플의 정량에 필요한 시약과 버퍼 및 항체가 코팅된 plate가 제공됩니다.
• 각 구성품들은 ELISA에 최적화 되어 있습니다.

• 제품 구성
  • Affinity purified antibody pre-coated 96 well ELISA microplate
  • Biotinylated affinity purified detection antibody
  • Lyophilized standard protein
  • Lyophilized color development enzyme (Streptavidin-HRP conjugate)
  • pink-ONE Assay Diluent
  • Substrate (pink-ONE TMB)
  • Stop solution
  • PBS powder
  • Tween-20 (50%)
  • Plate sealer

실험 원리

References
• Human TNF-alpha ELISA Kit (K0331131)
  Rosmarinic acid sensitizes cell death through suppression of TNF-[alpha]-induced NF-[kappa] B activation and ROS generation in human leukemia U937 cells DO Moon, MO Kim, JD Lee, YH Choi, GY Kim Cancer Lett. (2010) Feb 28;288(2):183-91 (abstract)
• Rat TNF-alpha ELISA Kit (K0331196)
  Subacute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranal AT Hariri, SA Moallem, M Mahmoudi, B Memar and H Hosseinzadeh Food and Chemical Toxicology, Volume 48, Issue 10, October (2010) Pages 2803-2808 (abstract)
• Mouse TNF-alpha ELISA Kit (K0331186)
  Regression of liver fibrosis by taurine in rats fed alcohol: Effects on collagen accumulation, selected cytokines and stellate cell activation Shanmugam Lakshmi Devia, Periyaswamy Viswanathanb and Carani V. Anuradha European Journal of Pharmacology, Volume 647, Issues 1-3, 25 November 2010, Pages 161-170 (abstract)

• Human IL-6 ELISA Kit (K0331194)
Hepatitis B Virus e Antigen Downregulates Cytokine Production in Human Hepatoma Cell Lines Shuang Wu, Tatsuo Kanda, Fumio Imazeki, Makoto Arai, Yutaka Yonemitsu, Shingo Nakamoto, Keiichi Fujiwara, Kenichi Fukai, Fumio Nomura, Osamu Yokosuka Viral Immunology. October (2010), 23(5): 467-476. doi:10.1089/vim.2010.0042.(abstract)
• Rat IL-6 ELISA Kit (K0331229)
Immunomodulatory effects of rosuvastatin on hepatic ischemia/reperfusion induced injury Azza S. Awad, Amany El Sharif Immunopharmacology and Immunotoxicology February 2, 2010.(abstract)
Regression of liver fibrosis by taurine in rats fed alcohol: Effects on collagen accumulation, selected cytokines and stellate cell activation Shanmugam Lakshmi Devia, Periyaswamy Viswanathanb and Carani V. Anuradha European Journal of Pharmacology, Volume 647, Issues 1-3, 25 November 2010, Pages 161-170 (abstract)
Investigation of antioxidant, anti-inflammatory and DNA-protective properties of eugenol in thioacetamide-induced liver injury in rats Baskaran Yogalakshmia, Periyasamy Viswanathanb and Carani Venkatraman Anuradha Toxicology, Volume 268, Issue 3, 9 February 2010, Pages 204-212 (abstract)
Genistein improves liver function and attenuates non-alcoholic fatty liver disease in a rat model of insulin resistance Sumiyabanu MOHAMED SALIH1, Palanisamy NALLASAMY1, Pooranaperundevi MUNIYANDI1, Viswanathan PERIYASAMI2, Anuradha CARANI VENKATRAMAN1 Journal of Diabetes, Volume 1, Issue 4, pages 278-287, December 2009 (abstract)
• Human IFN-gamma ELISA Kit (K0331121)
Lambs Infected with UV-Attenuated Sporocysts of Sarcocystis ovicanis Produced Abnormal Sarcocysts and Induced Protective Immunity against a Challenge Infection, Abdel-Azeem Abdel-Baki, Gamal Allam, Thabet Sakran, and El-Mahy El-Malah Korean J Parasitol. 2009 June; 47(2): 131-138 (PDF)
• Human sRANKL ELISA Kit (K0331187)
Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis Luca Dalle Carbonare, Maria Teresa Valenti, Mirko Zanatta, Luca Donatelli, Vincenzo Lo Cascio Arthritis & Rheumatism, Volume 60, Issue 11, pages 3356-3365, November 2009 (abstract)
• Human VEGF ELISA Kit (K0331132)
Comparative Anticancer Effects of Flavonoids and Diazepam in ultured Cancer Cells, Dae-Hyun KIM, Jae-Tae LEE, In-Kyu LEE, and Jeoung-Hee HA, Biol. Pharm. Bull. 31(2) 255-259 (2008) (pdf)
• Human GM-CSF ELISA Kit (K0331120)
Comparative Anticancer Effects of Flavonoids and Diazepam in ultured Cancer Cells, Dae-Hyun KIM, Jae-Tae LEE, In-Kyu LEE, and Jeoung-Hee HA, Biol. Pharm. Bull. 31(2) 255-259 (2008) (pdf)
• Mouse IL-4 ELISA Kit (K0331144)
Immunotoxicity of paraquat after subacute exposure to mice B. Riahia, H. Rafatpanahb, M. Mahmoudib, B. Memarc, A. Brookb, N. Tabasib and G. Karimi Food and Chemical Toxicology. Volume 48, Issue 6, June 2010, Pages 1627-1631 (abstract)
• Mouse EGF ELISA Kit, pink-ONE (K0331228P)
Epidermal growth factor increases insulin secretion and lowers blood glucose in diabetic mice H. Y. Lee1, K. Yea1, J. Kim1, B. D. Lee1, Y. C. Chae1, H. S. Kim1, D.-W. Lee4, S.-H. Kim2, J.-H. Cho1, C. J. Jin5, D.-S. Koh3, K. S. Park5, P.-G. Suh1, S. H. Ryu1 Journal of Cellular and Molecular Medicine, Volume 12, Issue 5a, pages 1593-1604 (abstract)
• Mouse IFN-gamma ELISA Kit (K0331138)
Epidermal growth factor increases insulin secretion and lowers blood glucose in diabetic mice H. Y. Lee1, K. Yea1, J. Kim1, B. D. Lee1, Y. C. Chae1, H. S. Kim1, D.-W. Lee4, S.-H. Kim2, J.-H. Cho1, C. J. Jin5, D.-S. Koh3, K. S. Park5, P.-G. Suh1, S. H. Ryu1 Journal of Cellular and Molecular Medicine, Volume 12, Issue 5a, pages 1593-1604 (abstract)
• Mouse MIP-2 ELISA Kit (K0131217)
Intestinal alkaline phosphatase contributes to the reduction of severe intestinal epithelial damage Marianne Bol-Schoenmakersa, , , Danielle Fiechtera, Willem Raabenb, Ine Hassinga, Rob Bleuminka, Danielle Kruijswijkb, Kelly Maijoorb, Monique Tersteeg-Zijderveldc, Ruud Brandsa and Raymond Pieters European Journal of Pharmacology, Volume 633, Issues 1-3, 10 May 2010, Pages 71-77 (abstract)
Syk kinase is required for collaborative cytokine production induced through Dectin-1 and Toll-like receptors, Kevin M. Dennehy, Gerben Ferwerda et al., Eur. J. Immunol. 2008. 38: 500.506 (pdf)
• Mouse IL-12 ELISA Core Kit, pink-ONE (K0131139P)
Surfactin inhibits immunostimulatory function of macrophages through blocking NK-κB, MAPK and Akt pathway Sun Young Parka and YoungHee Kim International Immunopharmacology, Volume 9, Issues 7-8, July 2009, Pages 886-893 (abstract)
• Mouse IL-13 ELISA Core Kit (K0131201)
Celastrol binds to ERK and inhibits FcεRI signaling to exert an anti-allergic effect Y Kim, K Kim, H Lee, S Han, YS Lee, J Choe, YM Kim, JH Hahn, JY Ro and D Jeoung European Journal of Pharmacology, Volume 612, Issues 1-3, 10 June (2009) Pages 131-142 (pdf)
• Human IgG ELISA Kit (K3231067)
Enhancement of therapeutic monoclonal antibody production in CHO cells using 30Kc6 gene Zesong Wanga, b, Ju Hyun Parka, Hee Ho Parka, Wensong Tanb and Tai Hyun Park Process Biochemistry March 2010 (abstract)

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