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 Á¦Ç°¾È³»  Assay Kit   Molecular Biology Kit  DNA Purification

EzWay¢â Genomic DNA/RNA Kit

¼¼Æ÷¿Í Á¶Á÷¿¡¼­ »Ó¸¸ ¾Æ´Ï¶ó ¹ÙÀÌ·¯½º, È¿¸ð, ½Ä¹°, Ç÷¾×µî¿¡¼­ genomic DNA¸¦ ¾òÀ» ¼ö ÀÖ´Â kit·Î, silica-based membraneÀ» ÀÌ¿ëÇÑ spin column¹æ½ÄÀ¸·Î ½ÇÇè °úÁ¤ÀÌ »¡¸® ÁøÇàµË´Ï´Ù.

KOMABIOTECH
Á¦Ç° ¹®ÀÇÇϱâ
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  • Silica-based membraneÀ» ÀÌ¿ëÇÑ spin column¹æ½ÄÀ¸·Î ½ÇÇè °úÁ¤ÀÌ ºü¸¨´Ï´Ù.
  • DNAÀÇ quality¿Í yield°¡ ³ô½À´Ï´Ù.
  • Phenol/chloroform ÃßÃâ°ú ¿¡Åº¿Ã ħÀüÀÌ ÇÊ¿ä¾ø½À´Ï´Ù.
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  • EzWay¢â Genomic DNA Kit, Multi (Animal/Bacteria/Yeast) (K11730, K11731)
    • µ¿¹°Á¶Á÷, ¹ÚÅ׸®¾Æ, °õÆÎÀ̷κÎÅÍÀÇ genomic DNA¸¦ ºü¸£°í °£ÆíÇÏ°Ô ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
      • Á¦Ç° ±¸¼º
      • Lysis Buffer A
      • Lysis Buffer B
      • Wash Buffer A
      • Wash Buffer B
      • Elution Buffer
      • Proteinase K
      • DNA-prep Column
      • 2ml Collection Tube
      • Elution Tube
      • Micropestle
  • EzWay¢â Genomic DNA Kit, Virus (K11738, K11739)
    • Plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, urineµîÀÇ body fluid·Î ºÎÅÍ viral nucleic acid¸¦ ºü¸£°í °£ÆíÇÏ°Ô ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
    • Á¦Ç° ±¸¼º
      • DNA-prep Column
      • Collection Tube
      • Lysis Buffer
      • Wash Buffer A
      • Wash Buffer B
      • RNase-Free Water
      • Carrier RNA
      • Elution Tube
  • EzWay¢â Genomic DNA Kit, Blood/Cultured Cells (K11740, K11741)
    • Whole blood cell ¹× Cultured cell·ÎºÎÅÍ genomic DNA¸¦ ºü¸£°í °£ÆíÇÏ°Ô ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
    • Á¦Ç° ±¸¼º
      • Lysis Buffer
      • Wash Buffer A
      • Wash Buffer B
      • Elution Buffer
      • Proteinase K
      • DNA-prep Column
      • 2ml Collection Tube
      • Elution Tube
  • EzWay¢â Genomic DNA Kit, Plant (K11742, K11743)
    • Plant cell·ÎºÎÅÍ genomic DNA¸¦ ºü¸£°í °£ÆíÇÏ°Ô ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
    • Á¦Ç° ±¸¼º
      • Suspension Buffer
      • Lysis Buffer
      • Binding Buffer
      • Wash Buffer A
      • Wash Buffer B
      • Elution Buffer
      • RNase A (50mg/ml)
      • Filter Column
      • DNA-prep Column
      • 2 ml Collection Tube
      • Elution Tube
    »ç¿ë¿¹
    Figure 2. EzWay¢â Genomic DNA Kit¿Í Ÿ»çÀÇ DNA Kit¸¦ ÀÌ¿ëÇÑ purified genomic DNAÀÇ ºñ±³

    Figure 1. EzWay¢â Genomic DNA Kit¿Í Ÿ»çÀÇ DNA Kit¸¦ ÀÌ¿ëÇÑ purified genomic DNAÀÇ ºñ±³

    Genomic DNA´Â Bacterial cell·ÎºÎÅÍ ºÐ¸®ÇÏ¿´À¸¸ç, Àü±â¿µµ¿Àº 0.7% TBE agarose gel¿¡¼­ ¼öÇàÇÏ¿´´Ù.

    M : DNA Marker
    1 : Ÿ»ç Á¦Ç°
    2 : EzWayTM Genomic DNA Kit, Bacteria

    Figure 3. Cell lysis °úÁ¤¿¡¼­ vortexing ¼ö¿¡ µû¸¥ E.coli·Î ºÎÅÍ ºÐ¸®µÈ genomic DNAÀÇ ºÐ¼®

    Figure 2. Cell lysis °úÁ¤¿¡¼­ vortexing ¼ö¿¡ µû¸¥ E.coli·Î ºÎÅÍ ºÐ¸®µÈ genomic DNAÀÇ ºÐ¼®

    Genomic DNA´Â E. coli·ÎºÎÅÍ ºÐ¸®ÇÏ¿´À¸¸ç, Àü±â¿µµ¿Àº 0.7% TBE agarose gel¿¡¼­ ¼öÇàÇÏ¿´´Ù.

    M : DNA Marker
    1 : vortexing 2¹ø
    2 : vortexing 3¹ø
    3~4 : vortexing 4¹ø
    5~6 : vortexing 6¹ø

    Figure 4. Hepatitis A virus »ùÇ÷κÎÅÍ ÁõÆøµÈ RT-PCR »ê¹°

    Figure 3. Hepatitis A virus »ùÇ÷κÎÅÍ ÁõÆøµÈ RT-PCR »ê¹°

    RT-PCR product´Â Hepatitis A virus·ÎºÎÅÍ ºÐ¸®ÇÏ¿© ÁõÆø ÇÏ¿´À¸¸ç, Àü±â¿µµ¿Àº 1.7% agarose gel¿¡¼­ ¼öÇàÇÏ¿´´Ù.

    M : DNA Marker (pBR322/Hinf¥°)
    C : Blank control

    Figure 5. EzWay¢â Genomic DNA Kit¿Í Ÿ»ç DNA Kit¸¦ ÀÌ¿ëÇÏ¿© Ç÷¾×À¸·Î ºÎÅÍ ºÐ¸®ÇÑ genomic DNA

    Figure 4. EzWay¢â Genomic DNA Kit¿Í Ÿ»ç DNA Kit¸¦ ÀÌ¿ëÇÏ¿© Ç÷¾×À¸·Î ºÎÅÍ ºÐ¸®ÇÑ genomic DNA

    Genomic DNA´Â Ç÷¾×À¸·Î ºÎÅÍ ºÐ¸®ÇÏ¿´À¸¸ç, Àü±â¿µµ¿Àº 0.7% agarose gel¿¡¼­ ¼öÇàÇÏ¿´´Ù.

    M : DNA Marker (1kb ladder)
    1, 2, 3 : Ÿ»ç kit¸¦ ÀÌ¿ëÇÏ¿© °¢°¢ 100ul, 200ul, 50ulÀÇ Ç÷¾×À¸·ÎºÎÅÍ ºÐ¸®
    4, 5, 6 : EzWay¢â Genomic DNA Kit¸¦ ÀÌ¿ëÇÏ¿© °¢°¢ 200ul, 100ul, 50ulÀÇ Ç÷¾×À¸·ÎºÎÅÍ ºÐ¸®

    Àû¿ë
  • PCR amplification, RT-PCR
  • Southern blotting
  • RAPD, AFLP and RFLP, etc
  • Membrane hybridizations
  • Restriction enzyme digestion