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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  Protein Research  Protein Assay Reagents

Protein assay reagents

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Cytoskeleton
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  • ´ÜÀÏ ±¸¼ºÇ° assay·Î »ç¿ë¹ýÀÌ °£´ÜÇÕ´Ï´Ù.
  • 1ºÐ ¾È¿¡ ´Ü¹éÁú ³óµµ¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ¾î ºü¸£°í, Á¤È®ÇÕ´Ï´Ù.
  • ¸Å¿ì ³·Àº ³óµµ¿Í ´Ù¾çÇÑ »ùÇÃÀÇ ³óµµ¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ƯÈ÷ ADV02´Â ÃøÁ¤ ¹üÀ§°¡ ³Ð½À´Ï´Ù.
  • °¢Á¾ detergent¿Í chemicalÀÌ Æ÷ÇÔµÈ »ùÇÿ¡µµ »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ´Ü¹éÁú ºÐ¼® reading¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â detergentÀÇ ÇÑ°è ³óµµ´Â ´ÙÀ½°ú °°½À´Ï´Ù.
  • Detergent ADV01 ADV02
    SDS 0.50% 0.10%
    NP40 0.20% 1.00%
    Triton X-100 0.20% 0.50%
    Tween 20 0.50% 1.00%
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  • Advanced Protein Assay (ADV01)
    • 25 ¥ìg/ml to 1000 ¥ìg/ml±îÁöÀÇ ´Ù¾çÇÑ ¹üÀ§ÀÇ ´Ü¹éÁú ³óµµ¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • °£´ÜÇÑ ¹æ¹ýÀ¸·Î 1ºÐ ¾È¿¡ green¿¡¼­ blue·Î »ö»óÀÌ º¯Çϸç 570~615nmÀÇ Èí±¤µµ¿¡¼­ ÃøÁ¤µË´Ï´Ù.
    • ´ÙÀ½°ú °°Àº »ùÇÿ¡ ÀÌ¿ëÇÒ¼ö ÀÖ½À´Ï´Ù.
      • SDS-PAGE sample buffer
      • Very low protein concentration solutions
      • Column fractions
      • Purified proteins and antibodies
  • Precision Red Advanced Protein Assay (ADV02)
    • 0.25 mg/ml¿¡¼­ 50 mg/ml±îÁöÀÇ ´Ù¾çÇÑ ¹üÀ§ÀÇ ´Ü¹éÁú ³óµµ¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • °£´ÜÇÑ ¹æ¹ýÀ¸·Î 1ºÐ ¾È¿¡ red¿¡¼­ blue·Î »ö»óÀÌ º¯Çϸç 600nmÀÇ Èí±¤µµ¿¡¼­ ÃøÁ¤µË´Ï´Ù.
    • ´ÙÀ½°ú °°Àº »ùÇÿ¡ ÀÌ¿ëÇÒ¼ö ÀÖ½À´Ï´Ù.
      • Cell extracts
      • Serum samples
      • Medium to high protein concentration solutions
      • Protein requiring GLP and higher standards
      • Purified proteins and antibodies
    »ç¿ë¿¹
    Figure 1: The low protein to protein variance with ADV01 and ADV02. A comparison of ADV01, ADV02, Bradford and Lowry assays on five different proteins.
    Figure 2. Comparison of protein measurements with ADV01 in PBS buffer and SDS-PAGE sample buffer. 2 ¥ìl of sample plus 300 ¥ìl of reagent were mixed in a well of a standard 96-well plate. Absorbance at 600 nm was read and plotted on the figure. ADV01 gives a linear response in both PBS and SDS-PAGE sample buffer. Note: The coefficient does change and measuremements in SDS-PAGE sample buffer should be compared to standards in the same buffer.
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
ADV01-AAdvanced Protein Assay (5X conc)1 x 500ml
ADV01-BAdvanced Protein Assay (5X conc)3 x 500ml
ADV02-APrecision Red Advanced Protein Assay (1X conc)1 x 500ml
ADV02-BPrecision Red Advanced Protein Assay (1X conc)3 x 500ml