ReBlot¢â Plus Antibody Stripping Solution & Recycling Kit
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membrane blot¿¡¼ Ç×ü¸¦ ¶¼¾î³»´Âµ¥ »ç¿ëÇÏ´Â ¿ë¾×À¸·Î, À̸¦ ÀÌ¿ëÇÏ¿© µ¿ÀÏÇÑ blot¿¡ ´ëÇØ ´Ù¾çÇÑ Ç×ü·Î ½ºÅ͵ð ÇÒ¼ö ÀÖ½À´Ï´Ù.
chemiluminescence, radioactive iodine, ȤÀº isotopesµîÀ» ÀÌ¿ëÇÑ western blot¿¡ »ç¿ëÇÒ¼ö ÀÖ½À´Ï´Ù. |
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| Merck Millipore |
- b-mercaptoethanol°¡ ÇÔÀ¯µÇ¾î ÀÖÁö ¾Ê¾Æ ³¿»õ°¡ ¾ø½À´Ï´Ù.
- Antibody strippingÀº heating¾øÀÌ ½Ç¿Â¿¡¼ 15ºÐ³»¿¡ °¡´ÉÇÕ´Ï´Ù.
- blotÀº 25ºÐÁ¤µµ ÈÄ¿¡ ¹Ù·Î ´Ù¸¥ Ç×ü·Î Àç»ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.
- 250-500 membrane strip ȤÀº 25-50 standard blot¿¡ ÀÌ¿ëÇÒ¼ö ÀÖ½À´Ï´Ù.
- Stripping °µµ¿¡ µû¶ó Strong°ú Mild typeÀ¸·Î Á¦°øµÇ¸ç, µÎ Á¦Ç°À» ¹¾î Recycling kitÀ¸·Î Á¦°øµÇ±âµµ ÇÕ´Ï´Ù.
Western blot probed with rabbit antibodies to peptide from angiotensin II type I receptor. 20 second exposure
Western blot stripped with ReBlot Plus Mild
Western blot reprobed with rabbit antibodies to peptide from glutamate receptor delta 1/2. 20 second exposure