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 제품안내  분자생물학   Genomic Research  DNA Research

AccuBlue dsDNA Quantitation Kit

dsDNA정량에 방해가 될수 있는 오염원의 영향을 받지 않고 정확히 dsDNA를 정량할수 있는 kit로, PicoGreen™이나 Quant-iT™ dsDNA detection kits에 비해 안정하고 편리합니다.
Biotium
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    특징
  • High sensitivity: well당 200 pg dsDNA (HS) 혹은 2 ng (BR) 정도까지 detection이 가능합니다.
  • Safety: 세포막을 투과하지 않으므로 안전하며, genotoxicity 염려를 없앴습니다.
  • Wide linear detection range: 0.2 - 100 ng (HS) 혹은 2 - 2000 ng (BR)의 dsDNA를 정량할수 있습니다.
  • Excellent selectivity: free nucleotides, proteins, detergents, salts 같은 일반적인 오염원과 RNA, ssDNA등으로부터 영향이 거의 없습니다.
  • Superior precision: Less than 5% CVs
  • Compatibility: microplate readers, spectrofluorometers, hand-held minifluorometers에 이용할수 있습니다.
  • Ex/Em: High Sensitivity kit: 485/530 nm; Broad Range kit: 350/460 nm
  • Storage efficiency: 4도에서 보관하므로 aliquot을 하지 않아도 되어 사용이 편리합니다.
  • Number of Assays: 1,000 with a 200 uL assay volume
    Kit의 구성
  • 1X aqueous dsDNA Quantitation Solution
  • enhancer
  • dsDNA standard (calf thymus)
사용법
MagicBlue™ dsDNA Quantitation Kit 사용법
    적용예
    Figure 1: Triplicate samples of calf thymus dsDNA, mouse liver RNA or M13mp18 viral ssDNA were assayed with the MagicBlue High Sensitivity dsDNA Quantitation Kit. Fluorescence was measured at 485/530 nm and plotted vs. the mass of the nucleic acids.
    Figure 1: Triplicate samples of calf thymus dsDNA, mouse liver RNA or M13mp18 viral ssDNA were assayed with the MagicBlue High Sensitivity dsDNA Quantitation Kit. Fluorescence was measured at 485/530 nm and plotted vs. the mass of the nucleic acids.
    Figure 2: MagicBlue can quantify a large number of dsDNA samples in four easy steps.
    Figure 2: MagicBlue can quantify a large number of dsDNA samples in four easy steps.
    Figure 3: Two-fold dilutions of calf thymus DNA were assayed using the MagicBlue HS kit with the QubitT™ fluorometer. Inset shows the lower end of the titration.
    Figure 3:Two-fold dilutions of calf thymus DNA were assayed using the MagicBlue HS kit with the QubitT™ fluorometer. Inset shows the lower end of the titration.
    Figure 4: Two-fold dilutions of calf thymus DNA were assayed using MagicBlue BR on a microplate reader. Inset shows the lower end of the titration.
    Figure 4: Two-fold dilutions of calf thymus DNA were assayed using MagicBlue BR on a microplate reader. Inset shows the lower end of the titration.
    Figure 5: MagicBlue High Sensitivity Reagent and PicoGreen stability was measured by reading absorbance of reagents in aqueous buffer at 5 min intervals at room temperature. The PicoGreen reagent decomposes quickly.
    Figure 5:MagicBlue High Sensitivity Reagent and PicoGreen stability was measured by reading absorbance of reagents in aqueous buffer at 5 min intervals at room temperature. The PicoGreen reagent decomposes quickly.
    Figure 6: Two-fold dilutions of calf thymus DNA were assayed using MagicBlue or Quant-iT Broad Range assay kits. MagicBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range.
    Figure 6: Two-fold dilutions of calf thymus DNA were assayed using MagicBlue or Quant-iT Broad Range assay kits. MagicBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range.
    Figure 7: The MagicBlue High Sensitivity reagent and PicoGreen were diluted to working concentrations in HeLa cell cultures and incubated for 30 minutes. No nuclear staining is evident with the MagicBlue reagent while PicoGreen readily binds nuclear DNA. Similar results were obtained with the Quant-iT High Sensitivity dsDNA reagent (Invitrogen).
    Figure 7: The MagicBlue High Sensitivity reagent and PicoGreen were diluted to working concentrations in HeLa cell cultures and incubated for 30 minutes. No nuclear staining is evident with the MagicBlue reagent while PicoGreen readily binds nuclear DNA. Similar results were obtained with the Quant-iT High Sensitivity dsDNA reagent (Invitrogen).