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 제품안내  면역학   Neuroscience  Ion Channel

Fluorescently Labeled Antibodies

오랫동안 형광성이 지속되는 ATTO dye가 라벨링된 항체와 FITC가 라벨링된 FACS용 항체를 소개합니다.
Alomone labs
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    ATTO dye labeled antibodies
  • 특징
    • strong absorption
    • high fluorescent quantum yield
    • high photo stability
    • IHC, ICC용으로 이용됩니다.
    • Green 형광을 띠는 ATTO-488과 Orange 형광을 띠는 ATTO-550이 있습니다.
    • Label Fluorescence Spectra Alternative to
    ATTO-488 Green ex 498nm, em 520nm in 0.1M phosphate pH7.0 FITC, Alexa-488
    ATTO-550 Orange ex 554nm, em 576nm in 0.1M phosphate pH7.0 TARMA, Cy3, Alexa-555

    flourescence label cat no. Product 용량
    atto-488 AAR-012-AG Anti-Angiotensin II Receptor Type-2 (AT2 Receptor) (extracellular)-ATTO-488 50ul
    atto-488 ACC-003-AG Anti-Cav1.2-ATTO-488 50ul
    atto-550 AQP-002-AO Anti-Aquaporin 2-ATTO-550 50ul
    atto-488 AGC-005-AG Anti-AMPA R2 (GluR2)-ext-ATTO 50ul
    atto-550 AAR-011-AO Anti-AT1 Receptor (ext.) ATTO-550 50ul
    atto-550 ANT-007-AO Anti-hp75ntr(ext)-ATTO-550 50ul
    atto-550 APR-004-AO Anti-P2X7-ATTO-550 50ul
    atto-550 ACC-063-AO Anti-STIM1(extra.)-ATTO-550 50ul
  • Expression of AT2 Receptor in mouse 3T3-L1 cells
    • Immunocytochemical staining of intact live mouse 3T3-L1 cells with Anti-AT2 Receptor (extracellular)- ATTO-488 antibody (#AAR-012-AG). green) (1:50). Live view of the same field was superimposed to the fluorescent one.
  • Expression of AT2 Receptor in rat brain
    • Immunohistochemical staining of AT2 receptor in rat brain. Rat frozen free floating sections were stained with Anti-AT2 Receptor (extracellular)-ATTO-488 antibody (#AAR-012-AG) (1:20). The T2 receptor (green) was detected in neurons in the vicinity of the hypothalamic paraventricular nucleus (PVN). In some neurons (thick white arrow), axonal processes with varicosities were observed (thin white arrow). Nuclei were visualized with DAPI counterstain (blue)
  • Expression of Aquaporin 2 in rat kidney
    • Immunohistochemical staining of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Aquaporin 2 is detected in collecting ducts but not in thin segments of the loop of Henle. Slides were treated with citrate for antigen retrieval and then incubated overnight at 4°C with Anti-Aquaporin 2-ATTO-550 antibody (#AQP-002-AO)(1:50) (Red). Nuclei are visualized with Hoechst 33342 (blue).
  • Expression of GluR2 in mouse cerebellum
    • Distribution of GluR2 in the molecular layer of the mouse cerebellum. Frozen free-floating sections were stained with Anti-AMPA Receptor 2 (GluR2)(extracellular)-ATTO-488 antibody (#AGC-005-AG) (1:20). Both dendrites of Purkinje cells (horizontal arrows) were stained (green). DAPI counterstain (blue) helps define the layers: granule (G), Purkinje (P), and molecular (M).
  • Expression of p75NTR in rat dorsal root ganglion (DRG) cells
    • Surface expression of p75NTR in rat dorsal root ganglion (DRG) cells. Intact live DRG neurons were labeled with Anti-mGluR1 (extracellular) antibody (#AGC-006) (1:100) followed by goat-anti-rabbit Alexa 488 secondary antibody (green). The cells were then labeled with Anti-human p75NTR (extracellular) ATTO-550 antibody #ANT-007-AO) (1:50) (red). Nuclei were visualized using the cell-permeable DNA binding dye Hoechst 33342 (blue). Note that the Anti-human p75NTR-(extracellular) ATTO-550 antibody does not stain all DRG neurons as expected. Colocalization is observed in some nerve fibers of the mGluR1 and p75NTR receptors.
    FITC labeled antibodies
  • 특징
    • Flow cytometry용으로 사용되는 FITC labeled antibody를 소개합니다.
    • flourescence label cat no. Product 용량
    FITC APC-109-F Anti-Kv11.1 (HERG) (extracellular) FITC 50ul
    FITC APC-101-F Anti-Kv1.3 (extracellular)-FITC 50ul
    FITC APR-008-F Anti-P2X7 (extracellular)-FITC 50ul
    FITC ACC-060-F Anti-Orai 1 (extracellular)-FITC 50ul
    FITC ACC-039-F Anti-TRPV2 (extracellular)-FITC 50ul
    FITC ANT-007-F Anti-Human p75NTR (extracellular)-FITC 50ul
    FITC AN-170-F Anti-rat p75-FITC 50ug
  • Anti-Kv 1.3(extracellular)-FITC
  • Anti-P2X7 (extracellular)-FITC
  • Anti-Human p75NTR-FITC
  • Anti-rat p75NTR-FITC