Hot Taq DNA Polymerase Kit

• Hot Taq DNA Polymerase, PCR buffer, dNTPs, Gel loading dye가 kit component로 제공됩니다.
• Modified Taq DNA Polymerase는 낮은 온도에서 형성된 primer-dimer와 nonspecifically annealed primers의 extension을 막아줍니다.
• background 없이 깨끗하고 nonspecific amplification이 감소된 정확한 결과를 얻을 수 있습니다.
• 높은 GC content를 가진 target의 증폭을 촉진시키는 Magic buffer가 함께 제공됩니다.
• Hot Taq concentration : 5U/ul
• Extension reate : 2-4kb/min at 72°C
• Amplification efficiency : ≥105 fold
• 5'→3' exonuclease activity : Yes
• 3'→5' exonuclease activity : No
• Extra A addition : Yes

제품구성
• Hot Taq DNA polymerase
• 10X Hot Taq PCR buffer
• dNTP Mixture
• Loading Buffer
• Magic Buffer (for high GC)

• Highly Specific PCR
• Low Copy Number Target PCR. (e.g. Viral Detection in Blood)
• RT-PCR of rare transcripts
• Differential Display
• Multiplex PCR, PCR-based DNA fingerprinting (VNTR, STR, and RAPD)etc
• Degenerate PCR

사용예
Figure 1. Human TNF gene amplification by serial dilution Hot Taq DNA polymerase and HotStarTaq DNA polymerase.

Lane 1 : 2.5 unit
Lane 2 : 1.2 unit
Lane 3 : 0.6 unit
Lane 4 : 0.3 unit
Lane 5 : 0.15 unit
Lane 6 : 0.08 unit
Figure 2. Low Copy Template DNA (HIV detection in human genomic DNA)

Lane 1 : 0 copy of HIV DNA in 50ng human gDNA
Lane 2 : 2 copies of HIV DNA in 50ng human gDNA
Lane 3 : 5 copies of HIV DNA in 50ng human gDNA
Lane 4 : 10 copies of HIV DNA in 50ng human gDNA
Lane M : 100bp ladder

• EzWay Hot Taq PCR MasterMix