Scepter¢â Handheld Automated Cell Counter
|
|
|
|
|
Scepter cytometer´Â Hemocytometer ¾øÀÌ ¼¼Æ÷¸¦ Ä«¿îÆÃÇÏ´Â ÆÄÀÌÆê ŸÀÔÀÇ ÈÞ´ë¿ë cell counter·Î, ¼¾¼¸¦ ÀÌ¿ëÇÏ¿©, ¼Ò·® »ùÇ÷κÎÅÍ ¼¼Æ÷ ¼ö¸¦ histogramÀ¸·Î È®ÀÎ ÇÒ ¼ö ÀÖ¾î »ç¿ë¹æ¹ýÀÌ ½±°í °£ÆíÇÕ´Ï´Ù. |
|
| Merck Millipore |
- ÆÄÀÌÆê ŸÀÔÀ̶ó, º¥Ä¡¿¡¼ ¹Ù·Î¹Ù·Î »ç¿ëÇÔÀ¸·Î½á, ÇöÀå¿¡¼ cellÀ» Áï½Ã counting ÇÒ¼ö ÀÖ½À´Ï´Ù.
- »ç¿ë Á¶ÀÛÀÌ °£´ÜÇϸç, ¼¾¼·Î cellÀ» »¡¾Æµé¿© ¾à 20ÃÊ Á¤µµ¸é cell counting °á°ú¸¦ º¼¼ö ÀÖ½À´Ï´Ù.
- ¼Ò·®(100 ul)»ùÇÿ¡¼ °á°ú È®ÀÎÀÌ °¡´ÉÇÕ´Ï´Ù.
- ÆÄÀÌÆê¿¡ ´Þ¸° ½ºÅ©¸°À» ÅëÇØ histogramÀ¸·Î ¹Ù·Î È®ÀÎ °¡´ÉÇÕ´Ï´Ù.
- Histogram °á°ú¹°À» 72°³±îÁö ÀúÀå °¡´ÉÇϸç, ÄÄÇ»ÅÍ·Î ÀڷḦ Àü¼ÛÇÏ¿© Ãß°¡ÀûÀ¸·Î ¿¢¼¿ÀÛ¾÷µµ °¡´ÉÇÕ´Ï´Ù.
- ´©±¸µçÁö ½±°Ô »ç¿ë °¡´ÉÇϸç, ½Ã°£°ú ³ë·ÂÀÌ Àý¾à µË´Ï´Ù.
- Á¤Àü±âÀûÀÎ ÀνÄÀ¸·Î ¼¾¼°¡ ÀÛµ¿ÇÕ´Ï´Ù.
- 6-36um »çÀÌÁîÀÇ cellÀº 60um sensor·Î, 3-17umÀÇ ÀÛÀº cellÀ̳ª particleÀº 40um sensor·Î ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
- Âü°í) 40um sensor¸¦ ÀÌ¿ëÇϱâ À§Çؼ´Â ±âÁ¸ Scepter 1.0 ¹öÀüÀº 2.0À¸·Î ¾÷±×·¹ÀÌµå µÇ¾î¾ß ÇÕ´Ï´Ù.

Sensor Specification
|
40 um Sensor |
60 um Sensor |
| Concentration Range of Sample |
50,000-1,500,000 particles/mL |
10,000-500,000 particles/mL |
Particle Diameter (operating range) |
3-17 microns |
6-36 microns |
Median Cell Diameter (recommended range) |
4-13 microns |
8-25 microns |

Sensor Selection Guide
| Type of Cell |
Measured size (¥ìm) |
40 ¥ìm |
60 ¥ìm |
| NIH 3T3 |
15 |
|
|
| 454 beads |
|
|
|
| Algae (various) |
7-9 |
|
|
| CHO |
14-17 |
|
|
| Cos-7 |
15 |
|
|
| Epithelia |
14-15 |
|
|
| HEK293 |
11-15 |
|
|
| HeLa |
12-14 |
|
|
| HepG2 |
12 |
|
|
| HT-29 |
11 |
|
|
| HUH7-Hepatoma line |
|
|
|
| B Cells |
6-11 |
|
|
| Human ES Cells |
9-12 |
|
|
| HUVEC |
14-15 |
|
|
| Jurkat |
13 |
|
|
| K562 |
22 |
|
|
| Luminex beads |
5-6 |
|
|
| MCF7 |
15-17 |
|
|
| MDCK |
13-15 |
|
|
| Mouse Embryomic Stem Cell |
5-13 |
|
|
| Mesenchymal Stem Cell |
15-16 |
|
|
| PBMCs |
7-12 |
|
|
| PC12 |
9-13 |
|
|
| Primary Astrocytes |
7 |
|
|
| Primary Neuronal Cell |
|
|
|
| Rat Whole Blood |
4.6 |
|
|
| Rat Dorsal Root Ganglion Cells |
7 |
|
|
| Red Blood Cells |
5-7 |
|
|
| Rat Neural Stem Cell |
11-13 |
|
|
| SF9 |
13 |
|
|
| SH-SY5Y |
12 |
|
|
| Splenocytes |
7-9 |
|
|
| U266 |
12 |
|
|
| U87-Human Glioblastoma Cell Line |
12-14 |
|
|
| Yeast-Pichia Pastoris |
5 |
|
|
| |
Recommended based on size |
|
Merck Millipore Validated |
|
Customer Validated |
- CellÀ» ½Å¼ÓÈ÷ counting ÇÒ ¶§
- ´Ù¾çÇÑ cellÀ» ÇѲ¨¹ø¿¡ check ÇÒ ¶§
- ÇÏ·ç¿¡ Çѹø ¾¿ ÀÚ¶ó´Â ¾çÀ» È®ÀÎ ÇÒ ¶§
- Ŭ¸°º¥Ä¡¿¡¼ °£´ÜÇÏ°Ô counting ¿øÇÒ ¶§

Á¦Ç°ÀÇ ±¸Á¶

Histogram

½ÇÇè ¿¹
- Immuno-monitoring
Figure 1. Differentiate Lymphocytes and Monocytes
We used the Scepter¢â 2.0 cell counter to rapidly determine lymphocyte and monocyte concentrations as well as the relative frequency of these cell types in peripheral blood mononuclear cell (PBMC) isolates.
In each case, three main peaks were distinguishable by each analysis method in the separation of lymphocytes from debris. The peaks correspond to lymphocytes (small cells), monocytes (large cells), and a debris/dead cell fraction.
- Cell Death
Figure 2. Qualitative Cell Health Assessments
when measured with the Scepter¢â Cell Counter, the treated cells showed a dramatic increase in the population of cells in the histogram to left of the main population. This indicated that there were larger populations of smaller size cells-likely representing the unhealthy cell populations.
- Cell Cycle
Figure 3. Assess Cell Cycle Changes
Size distribution profiles on the Scepter¢â Cell Counter shift to the right when treated with the mitosis blocking agent Colchicine, indicating cells are larger as they accumulate in G2/M phase. The Scepter¢â Cell Counter can be used as a cell culture monitoring device for a qualitative assessment of cell cycle disruption.
- Bead Counting
Figure 4. Accurate Bead Counting
Scepter counts beads with higher precision, as demonstrated by smaller coefficients of variation, compared to vision-based automated counting across multiple diverse bead types.
- Human PBMC Counting
Figure 5. Separate human PBMCs from whole blood
Our protocol for PBMC isolation enabled the generation of three distinct populations of blood cells, corresponding to RBCs, lymphocytes and monocytes. When the same PBMC samples were analyzed using flow cytometry (left) and a Scepter¢â cell counter (right), the data showed close agreement between the analytical techniques.
- Counting Yeast Cells
Figure 6. Count Yeast Cells during Fermentation
The Scepter¢â cell counter counts yeast cells with good accuracy and linearity. Measured yeast cell concentrations were compared to theoretical concentrations. The solid gray line represents the theoretical values. Dotted lines represent best linear fit to data. Both the Scepter¢â and Coulter Counter¢ç platforms show a loss of linearity and accuracy upon an increase in cell concentration.
- Adipogenesis Monitoring
Figure 7.
Using a 60 um sensor, the Scepter¢â cell counter enabled the discrimination of cell types based on size, with high resolution. ADSCs were measured at three key time points: Day 0 (control), seven (7) days after exposure to differentiation conditions, and fourteen (14) days differentiated. As indicated by the histogram data, cells gradually increased in size from 15 to 21 um over the fourteen-day differentiation.