Scepter™ Handheld Automated Cell Counter

• 파이펫 타입이라, 벤치에서 바로바로 사용함으로써, 현장에서 cell을 즉시 counting 할수 있습니다.
• 사용 조작이 간단하며, 센서로 cell을 빨아들여 약 20초 정도면 cell counting 결과를 볼수 있습니다.
• 소량(100 ul)샘플에서 결과 확인이 가능합니다.
• 파이펫에 달린 스크린을 통해 histogram으로 바로 확인 가능합니다.
• Histogram 결과물을 72개까지 저장 가능하며, 컴퓨터로 자료를 전송하여 추가적으로 엑셀작업도 가능합니다.
• 누구든지 쉽게 사용 가능하며, 시간과 노력이 절약 됩니다.
• 정전기적인 인식으로 센서가 작동합니다.
• 6-36um 사이즈의 cell은 60um sensor로, 3-17um의 작은 cell이나 particle은 40um sensor로 측정할 수 있습니다.
• 참고) 40um sensor를 이용하기 위해서는 기존 Scepter 1.0 버전은 2.0으로 업그레이드 되어야 합니다.

Sensor Specification

	40 um Sensor	60 um Sensor
Concentration Range of Sample	50,000-1,500,000 particles/mL	10,000-500,000 particles/mL
Particle Diameter (operating range)	3-17 microns	6-36 microns
Median Cell Diameter (recommended range)	4-13 microns	8-25 microns

Sensor Selection Guide

Type of Cell	Measured size (μm)	40 μm	60 μm
NIH 3T3	15
454 beads
Algae (various)	7-9
CHO	14-17
Cos-7	15
Epithelia	14-15
HEK293	11-15
HeLa	12-14
HepG2	12
HT-29	11
HUH7-Hepatoma line
B Cells	6-11
Human ES Cells	9-12
HUVEC	14-15
Jurkat	13
K562	22
Luminex beads	5-6
MCF7	15-17
MDCK	13-15
Mouse Embryomic Stem Cell	5-13
Mesenchymal Stem Cell	15-16
PBMCs	7-12
PC12	9-13
Primary Astrocytes	7
Primary Neuronal Cell
Rat Whole Blood	4.6
Rat Dorsal Root Ganglion Cells	7
Red Blood Cells	5-7
Rat Neural Stem Cell	11-13
SF9	13
SH-SY5Y	12
Splenocytes	7-9
U266	12
U87-Human Glioblastoma Cell Line	12-14
Yeast-Pichia Pastoris	5

Recommended based on size

Merck Millipore Validated

Customer Validated

• Cell을 신속히 counting 할 때
• 다양한 cell을 한꺼번에 check 할 때
• 하루에 한번 씩 자라는 양을 확인 할 때
• 클린벤치에서 간단하게 counting 원할 때

제품의 구조

Histogram

실험 예
• Immuno-monitoring
  

  Figure 1. Differentiate Lymphocytes and Monocytes
  We used the Scepter™ 2.0 cell counter to rapidly determine lymphocyte and monocyte concentrations as well as the relative frequency of these cell types in peripheral blood mononuclear cell (PBMC) isolates.
  In each case, three main peaks were distinguishable by each analysis method in the separation of lymphocytes from debris. The peaks correspond to lymphocytes (small cells), monocytes (large cells), and a debris/dead cell fraction.
• Cell Death
  

  

  Figure 2. Qualitative Cell Health Assessments
  when measured with the Scepter™ Cell Counter, the treated cells showed a dramatic increase in the population of cells in the histogram to left of the main population. This indicated that there were larger populations of smaller size cells-likely representing the unhealthy cell populations.
• Cell Cycle
  

  Figure 3. Assess Cell Cycle Changes
  Size distribution profiles on the Scepter™ Cell Counter shift to the right when treated with the mitosis blocking agent Colchicine, indicating cells are larger as they accumulate in G2/M phase. The Scepter™ Cell Counter can be used as a cell culture monitoring device for a qualitative assessment of cell cycle disruption.
• Bead Counting
  

  Figure 4. Accurate Bead Counting
  Scepter counts beads with higher precision, as demonstrated by smaller coefficients of variation, compared to vision-based automated counting across multiple diverse bead types.
• Human PBMC Counting
  

  Figure 5. Separate human PBMCs from whole blood
  Our protocol for PBMC isolation enabled the generation of three distinct populations of blood cells, corresponding to RBCs, lymphocytes and monocytes. When the same PBMC samples were analyzed using flow cytometry (left) and a Scepter™ cell counter (right), the data showed close agreement between the analytical techniques.
• Counting Yeast Cells
  

  Figure 6. Count Yeast Cells during Fermentation
  The Scepter™ cell counter counts yeast cells with good accuracy and linearity. Measured yeast cell concentrations were compared to theoretical concentrations. The solid gray line represents the theoretical values. Dotted lines represent best linear fit to data. Both the Scepter™ and Coulter Counter® platforms show a loss of linearity and accuracy upon an increase in cell concentration.
• Adipogenesis Monitoring
  

  Figure 7.
  Using a 60 um sensor, the Scepter™ cell counter enabled the discrimination of cell types based on size, with high resolution. ADSCs were measured at three key time points: Day 0 (control), seven (7) days after exposure to differentiation conditions, and fourteen (14) days differentiated. As indicated by the histogram data, cells gradually increased in size from 15 to 21 um over the fourteen-day differentiation.

관련 자료
• Scepter 한글 브로셔 Verson 1
• Scepter 한글 브로셔 Verson 2
• Scepter™ Cell Counter Performs
• Scepter Technology 브로셔

• Muse™ Cell Analyzer