EzWay¢â Direct ApoE Genotyping Kit
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Ç÷¾×¿¡¼ DNA purification °úÁ¤À» °ÅÄ¡Áö ¾Ê°í ¹Ù·Î Apolipoprotein E (ApoE) À¯ÀüÀÚÇüÀ» °Ë»çÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. |
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| KOMABIOTECH |
- One-step PCR·Î Apolipoprotein E (ApoE) genotyping¿¡ È¿°úÀûÀÎ Multiplex Kit ÀÔ´Ï´Ù.
- DNA¸¦ »ÌÁö¾Ê°í Ç÷¾×À¸·Î ¹Ù·Î genotypingÀ» Çϸç, PrimerMix¸¦ ÀÌ¿ëÇÏ¿© ´Ü 3½Ã°£¾È¿¡ °á°ú¸¦ º¼ ¼ö ÀÖ½À´Ï´Ù.
- 112¹ø (Cys/Arg)°ú 158¹ø (Arg/Cys) ¾Æ¹Ì³ë»êÀÇ º¯Çü¿¡ ÀÇÇØ »ý±â´Â ApoE 3°¡Áö ´ë¸³À¯ÀüÀÚÀÎ E2, E3, E4ÀÇ À¯ÀüÀÚ ´ÙÇü¼ºÀ» È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
- E2 (Cys112/Cys158), E3 (Cys112/Arg158), E4 (Arg112/Arg158)¿¡ ´ëÇÑ ApoE PrimerMix¸¦ Á¦°øÇÕ´Ï´Ù.
- Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye µîÀÌ È¥ÇյǾî ÀÖ´Â MasterMix ÇüÅ·ΠÁ¦°øµË´Ï´Ù.
- ApoE GenotypingÀ» ÅëÇØ ½ÉÇ÷°ü°èÁúȯ ¹× ¾ËÃ÷ÇÏÀ̸Ӻ´ÀÇ ¹ß»ý À§ÇèÀ» ¹Ì¸® ¿¹ÃøÇÒ ¼ö ÀÖ½À´Ï´Ù.
- ½ÇÇè°úÁ¤ÀÌ ´Ü¼øÇÕ´Ï´Ù.

Á¦Ç°±¸¼º
- 2X EzWay¢â Direct Taq PCR MasterMix
- 2.5X ApoE PrimerMix
- Size marker
- »ùÇÃÀ» ÁغñÇÑ´Ù.
- 2X EzWay Direct Taq PCR MasterMix ¿Í 2.5X ApoE PrimerMix¸¦ ÁغñÇÑ´Ù.
- 12.5ul EzWay Direct Taq PCR MasterMix, 10ul ApoE PrimerMix, 1.5ul distilled water, 1ul blood sampleÀ» tube¿¡ ³Ö°í Àß È¥ÇÕÇÑ´Ù. Negative controlÀº Ç÷¾×´ë½Å DW¸¦ ³Ö´Â°ÍÀ¸·Î ÁغñÇÑ´Ù.
- PCRÀ» ¼öÇàÇÑ´Ù.
- PCR »ê¹°À» 2% agarose gel¿¡ Àü±â¿µµ¿ÇÑµÚ ¿°»öÇÏ¿© ¹êµå¸¦ °üÂûÇÑ´Ù.
Direct ApoE genotyping from Blood
Ç÷¾×À» »Ì¾Æ PrimerMix¿Í È¥ÇÕÇÏ¿© ¹Ù·Î PCRÀ» ¼öÇàÇÔÀ¸·Î¼, ´Ü 3½Ã°£¾È¿¡ ApoEÀÇ À¯ÀüÀÚÇüÀ» º¼ ¼ö ÀÖ¾ú´Ù.