G-Protein Biochem Kit

• 각 G-Protein effector로 conjugation된 glutathione-sepharose (GST) bead를 이용하여 세포나 조직내 타겟 G-Protein을 침전시킵니다.
• 침전된 active G-Protein은 전기영동후, kit에서 제공되는 항체를 이용하여 Western Blotting으로 측정합니다.

종류 및 특징
• RhoA Activation Assay Biochem Kit (Cat# BK036)
  • 세포나 조직내 Activated RhoA를 측정할 수 있는 제품입니다.
  • GST-tagged Rhotekin-RBD protein을 이용하여 침전시킵니다.
  • 제품의 구성:
    • GST-tagged Rhotekin-RBD protein on colored agarose beads (Cat. # RT02)
    • RhoA-specific monoclonal antibody (Cat. # ARH01)
    • His-tagged RhoA protein (Cat. # RH01)
    • GTPγS: (non-hydrolyzable GTP analog) (Cat. # BS01)
    • GDP
    • Cell lysis Buffer
    • Wash Buffer
    • Loading Buffer
    • STOP Buffer
    • Protease inhibitor cocktail
  • 적용
    • Analysis of in vivo RhoA activation
  • 실험예
    • The RhoA activation assay was tested by loading the RhoA protein in cell lysates with either GTPγS or GDP. As expected, the GTPγS-loaded RhoA is very efficiently precipitated while very little GDP-loaded RhoA is precipitated (Fig. 1)
      

      Figure 1. Results from BK036 RhoA activation assay. Activated Rac was precipitated and detected in a Western blot using kit BK036. The first lane shows a 50 ng recombinant His-tagged RhoA standard (Rec. His-RhoA). The following lanes shows the pulldown of inactive, GDP-loaded, (RhoA-GDP PD) or active, GTPγS-loaded, Rac (RhoA-GTP PD) from equal amounts of cell lysates.
• Ras Activation Assay Biochem Kit (Cat# BK008)
  • 세포나 조직내 Activated Ras를 측정할 수 있는 제품입니다.
  • GST tagged Raf1-RBD protein을 이용하여 침전시킵니다.
  • 제품의 구성:
    • GST tagged Raf1-RBD protein bound to colored glutathione agarose beads (Cat. # RF02)
    • p21 Ras monoclonal antibody
    • Cell lysis Buffer
    • Protease inhibitor cocktail
    • Wash Buffer
    • Loading Buffer
    • STOP Buffer
    • GTPγS (non-hydrolyzable GTP analog, Cat. # BS01)
    • GDP
  • 적용
    • Analysis of in vivo RhoA activation
• Rac1 Activation Assay Biochem Kit (Cat# BK035)
  • 세포나 조직내 Activated Rac1을 측정할 수 있는 제품입니다.
  • GST-tagged PAK-PBD protein을 이용하여 침전시킵니다.
  • 제품의 구성:
    • GST-tagged PAK-PBD protein on colored agarose beads (Cat. # PAK02)
    • Rac1 monoclonal antibody (Cat. # ARC03)
    • His-tagged Rac1 protein (Cat. # RC01)
    • GTPγS: (non-hydrolyzable GTP analog) (Cat. # BS01)
    • GDP
    • Cell lysis Buffer
    • Wash Buffer
    • Loading Buffer
    • STOP Buffer
    • Protease inhibitor cocktail
  • 적용
    • Analysis of in vivo Rac1 activation levels.
    • Detection of compounds and proteins that enhance Rac1 activity.
    • Detection of compounds and proteins that inhibit Rac1 activity.
  • 실험예
    • The Rac1 activation assay was tested by loading the Rac1 protein in cell lysates with either GTPγS or GDP. As expected, the GTPγS-loaded Rac1 is very efficiently precipitated while very little GDP-loaded Rac1 is precipitated (Fig. 1)
      

      Figure 1. Results from BK034 Cdc42 activation assay. Activated Cdc42 was precipitated and detected in a Western blot using kit BK034. The first lane shows a 50 ng recombinant His-tagged Cdc42 standard (Rec. His-Cdc42). The following lanes shows the pulldown of iinactive, GDP-loaded, (Cdc42-GDP PD) or active, GTPγS-loaded, Cdc42 (Cdc42-GTP PD) from equal amounts of cell lysates.

• G-Protein G-LISA Kit