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Tubulin polymerization ¹× micotubule¿¡ °áÇÕÇÏ´Â ´Ü¹éÁú ¿¬±¸¿¡ »ç¿ëµÇ´Â Á¦Ç°ÀÔ´Ï´Ù. |
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| Cytoskeleton | |||
| Cat. No. | Tubulin | Detection Method |
|---|---|---|
| BK004 & CDS01 | >97% pure bovine | |
| BK006 & CDS03 | >99% pure bovine | |
| BK004P | >97% pure porcine | |
| BK006P | >99% pure porcine | |
| BK011 | >99% pure bovine | fluorescence based |
| BK011P | >99% pure porcine | fluorescence based |
| Assay Characteristics | Absorbance Assay | Fluorescence Assay |
|---|---|---|
| Tubulin used per assay | 300 ¥ìg | 100 ¥ìg |
| Volume of reaction | 100 ¥ìl | 50 ¥ìl |
| Signal to noise ratio | 2 | 4 |
| Coefficient of variation (cv)* | 13% | 11% |
| Paclitaxel EC50** | 1 ¥ìM | 1 ¥ìM |
| Vinblastine IC50 ** | 0.6 ¥ìM | 0.6 ¥ìM |
| Possible problems | Glycerol in standard assay format may interfere with drug or protein binding. Assay conditions can easily be altered to test this. | Fluorescent reporter may interfere with drug or protein binding. |





| Cat.NO | Product | Size | Add. |
|---|---|---|---|
| BK007R | Fluorescent microtubules Biochem Kit (Rhodamine) | 50-200 assays | ¹Ù±¸´Ï |
| BK027 | Kinesin/dynein motility Kit | 25 assays | ¹Ù±¸´Ï |
| BK029 | Micotubule binding protein spin-down Assay Kit | 30-100 assays | ¹Ù±¸´Ï |
| BK038 | Microtubule / tubulin in vivo Kit | 30-100 assays | ¹Ù±¸´Ï |
| BK015 | Microtubules/tubulin Biochem Kit | 8-200 assays | ¹Ù±¸´Ï |
| BK011 | Tubulin polymerization Assay (fluorescence) | 96 assays | ¹Ù±¸´Ï |
| BK011P | Tubulin polymerization Assay (fluorescence, >99% pure porcine tubulin) | 96 assays | ¹Ù±¸´Ï |
| BK006P | Tubulin polymerization assay Biochem kit (>99% pure isolated from porcine brain) | 24 assays | ¹Ù±¸´Ï |
| BK004 & CDS01 | Tubulin polymerization Assay Kit (>97% pure bovine tubulin) | 24 assays | ¹Ù±¸´Ï |
| BK004P | Tubulin polymerization Assay Kit (>97% pure porcine tubulin) | 24 assays | ¹Ù±¸´Ï |
| BK006 & CDS03 | Tubulin polymerization Assay Kit (>99% pure bovine tubulin) | 24 assays | ¹Ù±¸´Ï |