Phosphate Quantitation Kit

종류 및 특징
• ATPase & GTPase ELIPA kits (Cat# BK051, BK052)
  • 실시간적으로 hydrolysis동안 방출되는 phosphate의 양을 측정 할수 있습니다.
  • Enzyme Linked Inorganic Phosphate Assay 방법을 이용합니다.
  • 제품의 구성:
    • Reaction buffer
    • ELIPA reagent 1 (MESG)
    • ELIPA reagent 2 (PNP)
    • Phosphate standard
    • ELIPA reagent 1 resuspension buffer
    • ATP stock (Cat. # BSA04)
    • GTP stock (Cat. # BST06)
    • Manual with detailed protocols and extensive troubleshooting guide
  • 적용
    • Kinetic measurement of ATPase and GTPase activities
    • Discovery and characterization of ATPase/GTPase inhibitors
  • Product Citation
    • Webb, M.R. 1992. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proc. Natl. Acad. Sci. USA 89: 4884-4887.
    • Cheng Q., Wang Z-X., and Killilea SD. 1997. A continuous spectrophotometric assay for protein phosphatases. Analytical Biochemistry 226: 68-73.
• Kinesin HTS ATPase end point Assay (Cat# BK053)
  • kinesin motor proteins의 활성에 의한 hydrolysis동안 방출되는 inorganic phosphate (Pi)을 빠르고 경제적으로 측정할 수 있습니다.
  • colorimetric 방법을 이용합니다.
  • 제품의 구성:
    • CytoPhos™ reagent
    • Kinesin motor protein (Cat. # KR01)
    • Pre-formed microtubules (Cat. # MT001)
    • Kinesin Reaction Buffer
    • Paclitaxel (Cat. # TXD01)
    • Phosphate standard
    • DMSO
    • Manual with detailed protocols and extensive troubleshooting guide
  • 실험예
    • The activity of the Eg5-specific inhibitor, monastrol, was tested on 9 different kinesin motor domains (see the CMW page). Of the assayed motor domains, only the ATPase activity of Eg5 was inhibited. (Fig 1)
      
      Figure 1. Inhibition of kinesin activity with the Eg5 specific inhibitor monastrol. The results show percent of full kinesin activity in the presence of 100 μM monastrol. As expected, only Eg5 ATPase activity is inhibited by monastrol.
  • Product Citation
    • Goldstein, L. (1993) Annu. Rev. Genet. 27, 319-351
    • Sawin, K. E. and Scholey, J. M. (1991) Trends Cell Biol. 1, 122-129
    • Kuznetsov, S. A., and Gelfand, V. I. (1986) Proc. Natl. Acad. Sci. USA 83, 8530-8534
• CytoPhos phosphate Assay (Cat# BK054)
  • 제품의 구성:
    • CytoPhos™ reagent
    • Phosphate standard
    • Manual with detailed protocols and extensive troubleshooting guide
  • 적용
    • To measure phosphate in a biological solution or buffer
    • To measure ATP or GTP hydrolysis from an enzyme reaction
    • To measure phosphate release from phosphatase catalyzed reactions
• EasyRad phosphate Assay (Cat# BK055)
  • 제품의 구성:
    • Reaction buffer
    • Separator solution
    • ATP stock solution (Cat. # BSA04)
    • GTP stock solution (Cat. # BST06)
    • EasyRad™ Phosphate Assay Manual
  • 적용
    • To measure low activity ATPases or GTPases.
    • To measure phosphate release from other phosphatase catalyzed reactions.
• PhosFree phosphate Assay (Cat# BK050)
  • 제품의 구성
    • Activator
    • Quencher
    • 1 mM Phosphate Standard in dH2O
    • PhosFree™ Phosphate Assay Manual
  • 적용
    • To measure phosphate in a biological solution or buffer
    • To measure ATP or GTP hydrolysis from an enzyme reaction
    • To measure phosphate release from phosphatase catalyzed reactions
• Kinesin ELIPA™ Biochem Kit (Cat# BK060)
  • 제품의 구성:
    • Reaction buffer
    • ELIPA reagent 1 (MESG)
    • ELIPA reagent 2 (PNP)
    • Kinesin Heavy Chain motor domain protein (Cat. # KR01)
    • Pre-formed microtubules (Cat. # MT001)
    • Paclitaxel (Cat. # TXD01)
    • Phosphate standard
    • ELIPA reagent 1 resuspension buffer
    • ATP stock (Cat. # BSA04)
    • GTP stock (Cat. # BST06)
  • 적용
    • Kinetic measurement of microtubule activated kinesin ATPase activity
    • Discovery of kinesin inhibitors
    • Measurement of kinesin inhibitor IC50s