BlØk Noise Cancelling Reagents

• Ready-to-use 타입으로 사용이 편리합니다.
• Phosphoantibodies나 avidin-biotin systems이용시에도 non-specific binding을 감소시킬 수 있는 protein-free 시약입니다.
• SNAP id system에 validation되었습니다.
• 가지고 계시는 PVDF나 nitrocellulose membrane에 호환이 가능합니다.
• Bløk-CH reagent는 Immobilon HRP substrate와 호환이 가능합니다.
• 19장의 mini blot을 사용하는데 충분한 양이 제공됩니다. (8 x 7 cm)
• Bløk-CH/FL는 2년, Bløk-PO는 1년으로 보존기간이 깁니다.
• Detection 방법에 따라 3가지 제품중 원하시는 제품을 선택하여 사용할 수 있습니다.

제품의 종류
1. 1. Bløk-CH buffer: HRP chemiluminescence 또는 chromogenic detection시 효과적입니다.
2. 2. Bløk-FL buffer: fluorescence detection시 효과적입니다.
3. 3. Bløk-PO buffer: chemiluminescence나 fluorescence를 이용한 phosphoprotein Detection시 효과적입니다.

사용예
• Bløk-CH and Bløk-PO Reagents:
  Optimal background reduction while preserving phosphorylation state

Chemiluminescence detection of p53 in EGFstimulated A431 lysate (10 - 2.5 μg/lane, Millipore cat. no. 12-110).
Blots were blocked with Bløk-CH reagent, then probed with anti-p53 antibody (1:1,000, Millipore cat. no. AB565) diluted in Bløk-CH reagent.
Bands were detected using Luminata™ Forte Western HRP substrate (Millipore cat. no. WBLUF0500).
NFDM = Non-fat dry milk.

Chemiluminescence detection of pERK in EGFstimulated A431 lysate (10 - 2.5 μg/lane, Millipore cat.no. 12-110). Blots were blocked with Bløk-PO reagent, then probed with anti-pERK antibody (1:10,000, Millipore cat. no. 05-797R) diluted in Bløk-PO reagent.
Bands were detected using Luminata Forte Western HRP substrate (Millipore cat. no. WBLUF0500).
NFDM = Non-fat dry milk.
• Bløk-FL Reagent:
  Low background for fluorescence detection at 680 and 800 nm


Fluorescence detection of actin in EGF-stimulated A431 lysates (1.0 - 25 μg/lane, Millipore cat. no. 12-110). Lysates were resolved by SDS-PAGE and transferred onto Immobilon® FL transfer membrane (Millipore cat. no. IPFL00010). The membranes were blocked and probed with anti-actin antibody (1:400, Millipore cat. no. MAB1501) diluted in the respective blockers. Blots were then incubated with anti-mouse IgG IRDye® 800 (left panel) or 680 (right panel) conjugated (1:1,000, LI-COR cat. no. 926-32210), and scanned on the Odyssey® system after vacuum drying for 1 hr.

• EzWay™ DAB Western Blot Kit
• EzWay™ TMB Western Blot Kit
• Immobilon Western ECL HRP Substrate
• Luminata Western ECL HRP substrate