Feeder Cells
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hES cell의 파생과 유지 모두를 위해 필요한 Feeder cell입니다. 모든 Feeder cell은 사용하기 전 유사분열로 배양시켜야 합니다. |
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| Cell Biolabs |
MEF Feeder Cells
- Murine embryonic fibroblast (MEF) feeder cell은 undifferentiated 상태에서 hES cell의 유지를 위해 사용됩니다.
JK1 cells support the maintenance of murine embryonic stem cells. (A-B): Phase-contrast images (original magnification, x200) of murine ESC colonies on (A) JK1 cells and (B) mouse embryonic fibroblasts (MEFs) showed a similar, characteristic ESC morphology: tight, round colonies of cells with a high nucleus to cytoplasm ratio. (C-D): Immunohistochemistry (brown staining) showed expression of (C) NANOG, and (D) OCT4 in ESCs was maintained after culturing on JK1 cells for more than 1 month. (E-L): Immunofluorescence demonstrated that mESCderived teratomas expressed markers of all three germ layers: (E) CD31, (F) HNF3β, (G) mucin, (H) VE-cadherin, (I) NeuN, (J) GFAP, (K) Nestin, and (L) GCNA. Antibody staining is green. Nuclear counterstain is blue.
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