Platinum Retroviral Expression
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iPS cell (induced pluripotent stem cells) generationÀ» À§ÇÑ Retroviral expression kit À¸·Î, ¿ì¼öÇÑ packaging cell line°ú ´ÜÀÏ Çö󽺹̵å transfectionÀ¸·Î °í¿ª°¡(High-titer) ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ´Â vector ±â¼úÀ» ÅëÇÕÇϰí ÀÖ½À´Ï´Ù. |
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| Cell Biolabs |
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| Suitability of Retroviral Packaging Cell Lines by Host |
| - |
Plat-A Cells |
Plat-E Cells |
Plat-GP Cells |
| (Amphotropic) |
(Ecotropic) |
(Pantropic*) |
| Human |
+++ |
N.S. |
+++ |
| Mouse |
+++ |
+++ |
+++ |
| Rat |
+++ |
+++ |
+++ |
| Monkey |
+++ |
N.S. |
+++ |
| Cat |
+++ |
N.S. |
+++ |
| Dog |
+++ |
N.S. |
+++ |
| Hamster |
+ |
N.S. |
+++ |
| Bird |
N.S. |
N.S. |
+++ |
| Fish |
N.S. |
N.S. |
+++ |
| Frog |
N.S. |
N.S. |
+++ |
| Insect |
N.S. |
N.S. |
+++ |
| Mollusk |
N.S. |
N.S. |
+++ |
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*Retrovirus must be packaged with a pantropic envelope protein such as VSVG
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N.S. = Not Suitable
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Retrovirus Production in Packaging Cells and Virus Tropism