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 제품안내  분자생물학   PCR Amplification  Real Time PCR

Fast-Plus EvaGreen Master Mix

Fast-Plus EvaGreen Master Mix는 핵산 정량시 편리하게 바로 사용할 수 있는 hot-start master mix제품입니다.
Biotium
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    특징
  • PCR 에 매우 안정적인 dye 입니다.
  • PCR 에서 DNA와의 높은 결합력으로 정확한 melt 커브 곡선이 나타납니다.
  • hotstart Taq으로 활성화 되는데 약 2분밖에 소요되지 않습니다.
  • fast and regular cycling protocols에 모두 사용할 수 있습니다.
  • 다른 염색 dye를 넣을 필요 없이 전기영동을 통한 결과를 확인 할 수 있습니다.
    Selection guide
    Cat. No. Description Component PCR Instrument Size
    31020 Fast-Plus EvaGreen Master Mix (no ROX) EvaGreen® dye, dNTP, buffer composition(including Tris and MgCl2) and CheetahTM hot-start Taq polymerase. Optimized for most of the non-ABI instruments which do NOT require ROX reference dye. BioRad: iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384, MJ Opticon, Option2, Chromo4, MiniOpticon
    Qiagen: Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000
    Eppendorf: Mastercycler realplex
    Illumina: Eco RealTime PCR System
    Cepheid: SmartCyler
    Roche: LightCycler 480, LightCycler 2.0     		200 rxn (2X1 mL)
    31020-1 500 rxn(5X1 mL)
    31020-2 5,000 rxn(50X1 mL)
    31014 Fast-Plus EvaGreen Master Mix with Low ROX EvaGreen® dye, dNTP, buffer composition (including Tris and MgCl2), CheetahTM hot-start Taq polymerase and low concentration of ROX reference dye. ABI: 7500, 7500 Fast
    Stratagene: MX4000P, MX3000P, MX3005P     		200 rxn (2X1 mL)
    31014-1 500 rxn(5X1 mL)
    31014-2 5,000 rxn(50X1 mL)
    31015 Fast-Plus EvaGreen Master Mix with High ROX Evagreen® dye, dNTP, buffer composition(including Ris and MgCl2), CheetahTM hot-start Taq polymerase and high concentration of ROX reference dye. ABI: 5700, 7000, 73000, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne pluse 200 rxn (2X1 mL)
    31015-1 500 rxn(5X1 mL)
    31015-2 5,000 rxn(50X1 mL)
    적용
    Unrivaled PCR Performance

    Figure 1. Comparison among Fast-Plus EvaGreen® master mix from Biotium and two fast SYBR Green master mixes from two leading companies (company A and company Q) under similar conditions. The inset is an enlarged view of the area near the baseline for better viewing the curve patterns of the much weaker signals of the two SYBR-based master mixes. Amplicon: ATPG fragment of human genomic DNA; instrument: ABI 7900 Fast.
    Cell impermeability of EvaGreen® dye makes it the only PCR dye to date that is non-mutagenic, non-cytotoxic and safe to aquatic life

    Figure 2. Comparison of cell membrane permeability between EvaGreen® dye and SYBR® Green I. HeLa cells were incubated with SYBR® Green I (1.2 uM) or EvaGreen® dye (1.2 uM) at 37 °C. Photographs were taken following incubation for 5 and 30 minutes. SYBR® Green I entered cells rapidly while EvaGreen® dye appeared membrane-impermeable as evident from the absence of cell nuclear staining. Image taken with long photo-exposure time revealed that EvaGreen® dye only associated with cell membranes. SYBR® Green I has been suggested to interfere with the DNA repair mechanism in living cells, a rationale used to explain the observation that the dye is even more environmentally toxic than ethidium bromide (Ohta, et al. Mutation Research, 492, 91-97(2001)). In contrast, EvaGreen® dye has been confirmed to be non-mutagenic and non-cytotoxic.