G-switch™ Activators and Inhibitors

• endogenous Rho family members의 강력한(5~10배) activator입니다.
• 타겟 단백질의 over-expression이나 knockdown에 의존하는 기존 방법과는 달리 G-switch™의 시약은 반응을 생성하는 기회를 최적화시켜주고, 2-4시간 안에 activation됩니다.
• multiple cell type에도 효과적입니다.

Dose-dependent activation of RhoA in Swiss 3T3 cells using CN03.

Actin cytoskeleton morphological changes induced by CN03 treatment of Swiss 3T3 cells. Swiss 3T3 fibroblasts plated on glass coverslips, grown for 2 days in DMEM plus 10% FBS, serum starved for 24 h in media containing 1% FBS followed by 24 h in serum free media. Cells were treated with a buffer control (A) or 1 u/ml CN03 for 2 h at 37°C/5% CO2. Cells were then fixed, stained with Acti-stain™ 488 phalloidin (Cat.#PHDG1), and visualized by fluorescence microscopy. Images were taken at a magnification of 40x. The control cells (A) exhibited mainly edge ruffling, some lamellipodia and very few stress fibers, whereas treatment with CN03 (B) resulted in the development of abundant stress fibers with some edge ruffling. Under similar conditions, RhoA was activated ~10-fold as determined using the RhoA G-LISA® activation assay (Cat.# BK124).

• FtsZ protein
• Tubulin & Microtubules