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ProSci immunoBlots

´Ù¾çÇÑ Á¾·ùÀÇ cell lineÀ̳ª Á¶Á÷¿¡¼­ Ç×ü¿Í ´Ü¹éÁú ¹ßÇöÀ» ½ºÅ©¸®´×ÇÒ ¼ö ÀÖ´Â immunoblot Á¦Ç°À» ¼Ò°³ÇÕ´Ï´Ù.

ProSci
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  • human, mouse, ratÀÇ Á¶Á÷ »Ó¸¸ ¾Æ´Ï¶ó Á¾¾ç Á¶Á÷°ú cell line¿¡¼­ ´Ù¾çÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ´Ü¹éÁúÀ» 4-20% gradient SDS-PAGE gelÀ» ÀÌ¿ëÇÏ¿© ºÐ¸®Çϰí, nitrocellulose membraneÀ¸·Î trasfer ½ÃÄÑ ¹Ù·Î immunoblot ÇÒ ¼ö ÀÖµµ·Ï Á¦°øµÇ¾îÁý´Ï´Ù.
  • °¢ Immunoblot¿¡´Â pre-stained protein marker¿Í 8-9°³ÀÇ »ùÇÃ(»ùÇà ´ç 15 ¥ìg ´Ü¹éÁú)ÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
  • ¿äû½Ã 7% ~ 15% gelÀÇ »ç¿ëÀÌ °¡´ÉÇϸç, ¸ÂÃãÇü immunoblotµµ »ý»ê °¡´ÉÇÕ´Ï´Ù.
Coomassie blue staining of human cell lysates in 12% SDS-PAGE.
Coomassie blue staining of human cell lysates in 12% SDS-PAGE.
Lanes:
1. protein markers
2. MCF7
3. HeLa
4. HT-29
5. 293
6. Hep G2
7. A-549
8. SK-N-SH
9. PC-3
    Á¦Ç°ÀÇ Á¾·ù
    Cat. No. Product Description Aplication
    1501 Human Cell Line I Blot MCF, HeLa, HT-29, 293, Hep G2, A-549, SK-N-SH, PC-3 WB
    1502 Human Cell Line II Blot Daudi, Raji, K-562, U-937, THP-1, HL-60, Jurkat, MOLP-4 WB
    1511 Human Lung Tumor Blot II A549, small, non-small, squamous cell carcinoma, matched normal lysates WB
    1513 Human Lung Tumor Blot IV A549, small cell, differentianted carcinoma, pleomorphic carcinoma, matched normal lung tissue WB
    1514 Human Lung Tumor Blot V A549, small cell, differentianted carcinoma, pleomorphic carcinoma, matched normal lung tissue WB
    1546 Human Lung Tumor Blot VI A549, normal lung tissue, small cell, non-small cell, squamous, pleo-morphic, non-diagnosed carcinoma, differentiated adenocarcinoma WB
    1521 Human Normal Tissue Blot I brain, colon, heart, kidney, liver, lung, pancreas, spleen WB
    1522 Human Normal Tissue Blot II brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, spleen WB
    1523 Human Normal Tissue Blot III colon, duodenum, esophagus, ileum, small intestine, jejunum, pancreas, rectum, stomach WB
    1524 Human Normal Tissue Blot IV bladder, breast, cervix, kidney, ovary, placenta, prostate, testis, uterus. WB
    1525 Human Normal Tissue Blot V appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, thyroid WB
    1526 Human Normal Tissue Blot VI left & right cerebellum, frontal & occipital & pariet lobe, whole eye, spinal cord, temporal lobe, thalamus. WB
    1542 Human Tumor Tissue Blot I brain, colon, kidney, liver, lung, pancreas, skin, spleen, stomach WB
    1543 Human Tumor Tissue Blot II colon, duodenum, esophagus, small intestine, liver, pancreas, rectum, stomach WB
    1544 Human Tumor Tissue Blot III bladder, breast, kidney, ovary, prostate, testis, cervix, uterus WB
    1545 Human Tumor Tissue Blot IV kidney, liver, lymphoma, Non-Hodgkin's lymphoma, spleen, thymoma, thyroid, tonsil WB
    1561 Mouse Normal Tissue Blot I brain, colon, heart, kidney, liver, lung, pancreas, spleen WB
    1562 Mouse Normal Tissue Blot II adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, thymus WB
    1581 Rat Normal Tissue Blot I brain, colon, heart, kidney, liver, lung, pancreas, spleen WB
    1582 Rat Normal Tissue Blot II adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, thymus WB