Nuclear/Cytosolic Fractionation Kit

• 2시간 이내에 추출이 가능하며 회수율이 높고, 교차 오염이 적습니다.
• 추출된 단백질은 유전자지문분석, EMSA, 효소활성분석, Western blotting 등의 실험에 사용이 가능합니다.
• 세포기질 분획 및 핵 단백질 추출이 가능합니다.
• 각 Prep당 5 x 10⁶개의 세포를 사용할 수 있습니다.

Kit 구성품
• Cytosol Extraction Buffer, Hypotonic
• Cell Lysis Reagent
• Nuclear Extraction Buffer
• Dithiothreitol (1000X)
• Protease Inhibitor Cocktail (100X)

사용 예

Figure 1: HEK293 Cell Fractionation.

Cytosolic and nuclear protein extracts were isolated from Human Embryonic Kidney 293 cells according to the Assay Protocol. Whole cell (W), cytosol (C), and nuclear (N) fractions were immunoblotted with Anti-α-Tubulin (left) or Anti-Lamin A/C (right) at 1 μg/mL.

Figure 2: HEK293 Trypan Blue Staining.

Human Embryonic Kidney 293 cells were stained with Trypan Blue at various steps during the fractionation protocol, demonstrating complete lysis and high neuclei recovery.

• AccuBlue dsDNA Quantitation Kit
• Restriction Enzyme