Bacterial Protein Extraction Reagents

• Sonication 등의 기계적인 방법을 사용하지 않고 E.coli로 부터 재조합 단백질을 쉽고 빠르게 추출할 수 있습니다.
• 단일 step으로 높은 회수율을 가지며, 총 30분 이내에 추출이 가능합니다.
• 소규모 혹은 대량의 단백질 추출이 가능합니다.

Bacterial Protein Extraction Flow Chart

사용 예

Figure 1: Extraction of His-RFP fusion protein.

His-RFP expression in BL21 was induced with 1 mM IPTG at 37ºC for 4 hrs. Cells were pelleted (left), then resuspended in Bacterial Protein Extraction Reagent for 15 minutes, according to the assay protocol. The supernatant (right) containing the extracted His-RFP was collected from the insoluble post-extraction pellet (center).

Figure 2: Comparison of Sonication with Bacterial Protein Extraction Reagent.

Pelleted His-RFP cells were sonicated in PBS/Triton X-100 or resuspended in Bacterial Protein Extraction Reagent for 15 minutes. Lysates were then collected and tested for RFP fluorescence on a 96-well fluorometer.

• EzWay™ PAG Protein Elution Kit
• GeBA flex-tube Kit
• ProteoExtract® Cytoskeleton Enrichment Kit