RAPAd¢ç Adenoviral Expression Systems
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´Ü 2ÁÖ À̳»¿¡ Wild-typeÀÇ Adonovirus´Â °¨¼Ò½ÃŰ¸é¼ Recombinant Adenovirus¸¦ »ý»êÇÏ´Â ½Ã½ºÅÛÀÔ´Ï´Ù. |
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| Cell Biolabs |
- Packaging signal°ú E1 sequence°¡ Á¦°ÅµÈ 5' ITR·ÎºÎÅÍ ¾òÀº Backbone vector¸¦ »ç¿ëÇÕ´Ï´Ù.
- ´ëºÎºÐÀÇ ´Ù¸¥ ½Ã½ºÅÛº¸´Ù 100-10000¹è ´õ ÀûÀº WT plaques¸¦ »ý»êÇÏ¿©, ½ÇÁúÀûÀ¸·Î Wild type Adenovirus°¡ Á¸ÀçÇÏÁö ¾Ê½À´Ï´Ù.
- 2-3°³¿ùÀÌ °É¸®´Â ´Ù¸¥ ¹æ¹ý°ú´Â ´Þ¸® 2-3ÁÖ À̳»¿¡ ÀçÁ¶ÇÕ ¹ÙÀÌ·¯½º¸¦ ¾òÀ¸½Ç ¼ö ÀÖ½À´Ï´Ù.
- RSV, CMV µî °ü½ÉÀÖ´Â À¯ÀüÀÚ Ç¥ÇöÀ» À§ÇÑ Promoter¿¡ µû¶ó Kit¸¦ ¼±ÅÃÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
Figure 1. Adenovirus Production using the RAPAd¢ç Adenoviral Expression System.