ProteoExtract® Cytoskeleton Enrichment Kit

• 20분 이내에 Western blot, Mass spectrometry 혹은 Imaging 분석에 사용하는 cytoskeleton 및 연관된 단백질을 빠르고 간편하게 농축합니다.
• 농축된 cytoskeleton fraction의 회수율을 증가시킵니다.
• Actin cytoskeleton 및 관련 단백질의 부착 형태 등 있는 그대로의 특성을 유지시킵니다.
• 엄격한 분획 방법을 이용하여 회수율이 낮은 cytoskeleton 관련 단백질의 검출을 향상시켜 주고, Background signal을 최대한 줄여줍니다.

종류

Cat. No.	Product	용도	구성품
17-10195	ProteoExtract® Cytoskeleton Enrichment & Isolation Kit	다양한 세포에서 Cytoskeleton 및 그와 연관된 단백질을 농축하고 분리합니다.	10x Cellular Extraction Buffer
			20x Cytoskeleton Wash Buffer
			Nuclear Extraction Buffer
			Protease Inhibitor Cocktail
			Sodium Orthovanadate Solution
			Cytoskeleton Solubilization Buffer
			Goat anti-Mouse IgG HRP Conjugate
			Anti-GAPDH Mouse Monoclonal Antibody
			Anti-Vimentin Mouse Monoclonal Antibody
17-10210	ProteoExtract® Native Cytoskeleton Enrichment Kit	전세포를 면역형광염색하여 관찰하기 위해 cytoskeleton 및 관련 단백질을 농축하고 염색하는 제품입니다.	10x Cellular Extraction Buffer
			20x Cytoskeleton Wash Buffer
			Nuclear Extraction Buffer
			Protease Inhibitor Cocktail
			Sodium Orthovanadate Solution.
			Donkey anti-Mouse IgG, FITC Conjugate
			10x Blocking/Permeabilization Buffer
			TRITC-Conjugated Phalloidin
			DAPI
			Anti-Vinculin Mouse Monoclonal Antibody
			Anti-GAPDH Mouse Monoclonal Antibody

Figure 1. Western Blot of compartmental proteins extracted from HeLa cells using ProteoExtract Cytoskeleton Enrichment and Isolation Kit (cat. # 17-10195).

Non-Enriched		Enriched

Figure 2. Improved visualization of actin-associated proteins with reduced background (right) after enrichment of cytoskeletal proteins. Confocal fluorescence microscopy of non-enriched and enriched HeLa cells. Background due to soluble cytosolic fraction was significantly reduced after enrichment using the ProteoExtract® Cytoskeleton Enrichment and Staining Kit (Cat. # 17-10210), resulting in clear detection of insoluble, actin-associated proteins (vinculin).

• Bacterial Protein Extraction Reagents
• EzWay™ PAG Protein Elution Kit
• GeBA flex-tube Kit