• Home
  • About us
  • Log-In
  • Join Us
  • Sitemap
  • Recruit
제품/실험 Q&A
실험기법
자료신청
클레임 접수
기기 A/S 접수
클레임 진행조회
 제품안내  분석 제작 서비스   신약개발 탐색 서비스

Lipidomics 분석서비스 (Lipidomics Analysis Service)

Lipids는 세포내 필수적 기능을 지원하는 세포막의 기반을 형성할 뿐만 아니라, 대사, 호르몬, 필수 영양소 또는 세포신호분자로서 작용하는 small molecules입니다. 광범위 lipids에 대한 정량분석 (Lipidomics analysis)은 다양한 질환에서의 lipid 바이오마커와 신약후보 탐색하는데 가장 빠르고 효과적입니다. Lipidomics 분석 data는 생명공학 및 제약 , 피부과학 및 화장품 개발, 식품산업 등 다양한 분야에서 lipid biology에 대한 새로운 insight를 제공합니다.

Lipotype
제품 문의하기
인쇄하기




  • 세포, 조직, 장기, 혈액, 미생물, 피부각질 등 다양한 시료 내 존재하는 lipid를 분석해 드립니다.
  • Shotgun Mass 분석방법으로 여러 다양한 lipid를 한번에 측정할 수 있습니다.
  • High resolution instrument를 이용하며 GMP 인증랩에서 진행됩니다.
  • 모든 지질을 한번에 측정함으로써 포괄적인 통계 분석이 가능합니다. (Lipidome)

 광범위 분석커버리지 (ultra-broad coverage)
  • 3100종 이상의 individual lipids에 대해 57 lipid classes 및 species 또는 subspecies 레벨에서 정량분석합니다.
  • 예) PC (lipid class)> PC 34:0 (species) > PC 18:0_16:0 + fatty acid information (subspecies)




다양한 시료의 lipidome분석
  • Tissues and organs (liver, brain, etc.) 
  • Cultured cells 및 세포 소기관 (subcellular compartments) 
  • Body fluids, blood and plasma
  • Bacteria, yeast 등 microorganisms
  • Skin tapes

 

Shotgun Mass Spectrometry기술 기반의 high-throughput quantitation
  • 고해상도 mass 분석장비인 Lipotype Explorer를 이용하여 소량의 (small amount) 시료를 단 시간에 high-throughput으로 정량분석합니다. 
  • 시료의 추출부터 processing, data 분석까지 자동화된, GMP인증을 받은 shotgun lipidomics 분석기술은 대량의 시료분석에도 안정적이고, 재현성이 높은 분석결과를 제공합니다.

 

LipotypeZoom을 이용한 통계적 분석결과 제공
  • 모든 시료에 대해 해당 lipid class마다 특정 internal standard를 사용하여 정량분석하므로 제공되는 data는 절대값 (absolute value)으로 제공됩니다. 
  • 분석한 시료에 대해 이해할 수 있는 통계자료로 정보를 변환하여 제공합니다.
    - Standard report (기본제공): lipid analysis data (pmole and mol% data for every sample) 및 수치를 그래프화한 보고서 
    - Extended report (선택제공): LipotypeZoom에 직접 접근할 수 있는 계정을 제공하여 원하는 모든 종류의 통계자료를 필요에 따라 도출가능

 

   * Lipid class 분석



   * 코흐트 분석



   * HeatMap분석



    * PCA분석







 

  Standard Analysis Premium Analysis
Delivery time 6 weeks 4 weeks
Minimum 15 samples 20 samples
Structural Details Glycerophospholipids and glycerolipids (except TAG and CL) are reported as subspecies – e.g. PC-36:1;0(18:0;0-18:1;0) denotes PC with the total length of its fatty acids of 36, 1 double bond and 0 hydroxylations that is made up from two fatty acids: 18:0 (stearic) and 18:1 (oleic). The remaining lipid classes are reported as species e.g. – CER-34:1;2. Glycerophospholipids and glycerolipids (except TAG and CL) are reported as subspecies – e.g. PC-36:1;0(18:0;0-18:1;0) denotes PC with the total length of its fatty acids of 36, 1 double bond and 0 hydroxylations that is made up from two fatty acids: 18:0 (stearic) and 18:1 (oleic). The remaining lipid classes are reported as species e.g. – CER-34:1;2.
Approach LipotypeXplorer identifies all measurable lipids in a sample using molecular fragmentation query language without the need to rely on predefined databases. LipotypeXplorer identifies all measurable lipids in a sample using molecular fragmentation query language without the need to rely on predefined databases.
Results Quantitative
Results are expressed in absolute values - pmol per sample volume.
Quantitative
Results are expressed in absolute values - pmol per sample volume.
Standard report contains:
Information on methodology, analysis details, used sample amounts, technical variation and quantitative lipidomics results in tabular format.
contains:
Information on methodology, analysis details, used sample amounts, technical variation and quantitative lipidomics results in tabular format.
Basic coverage PA - phosphatidate
PC - phosphatidylcholine
PE - phosphatidylethanolamine
PG - phosphatidylglycerol
PI - phosphatidylinositol
PS - phosphatidylserine
DAG - diacylglycerol
TAG - triacylglycerol
SM - sphingomyelin•
CE - cholesteryl ester•
EE - ergosteryl ester••
• - animals
•• - yeast
PA - phosphatidate
PC - phosphatidylcholine
PE - phosphatidylethanolamine
PG - phosphatidylglycerol
PI - phosphatidylinositol
PS - phosphatidylserine
DAG - diacylglycerol
TAG - triacylglycerol
SM - sphingomyelin•
CE - cholesteryl ester•
EE - ergosteryl ester••
• - animals
•• - yeast
Extended coverage LPA - lyso-phosphatidate
LPC - lyso-phosphatidylcholine
LPE - lyso-phosphatidylethanolamine
LPG - lyso-phosphatidylglycerol
LPI - lyso-phosphatidylinositol
LPS - lyso-phosphatidylserine
PC O- - ether-linked PC•
PE O- - ether-linked PE•
LPC O- - ether-linked LPC•
LPE O- - ether-linked LPE•
CER - ceramide
HEXCER - hexosylceramide•
CL - cardiolipin
• - animals
LPA - lyso-phosphatidate
LPC - lyso-phosphatidylcholine
LPE - lyso-phosphatidylethanolamine
LPG - lyso-phosphatidylglycerol
LPI - lyso-phosphatidylinositol
LPS - lyso-phosphatidylserine
PC O- - ether-linked PC•
PE O- - ether-linked PE•
LPC O- - ether-linked LPC•
LPE O- - ether-linked LPE•
CER - ceramide
HEXCER - hexosylceramide•
CL - cardiolipin
• - animals
Sample amount adjustment Samples are pre-tested to ensure that the most optimal amounts are used for analysis. Samples are pre-tested to ensure that the most optimal amounts are used for analysis.
Free sterols coverage N/A Available (extra charge)
Free cholesterol or ergosterol (y)
*y - yeast only
Yeast specific coverage N/A IPC - inositolphosphorylceramide
MIPC - mannosyl-inositol phosphorylceramide
M(IP)2C - mannosyl-di-(inositolphosphoryl) ceramide
CDP-DAG - CDP-diacylglycerol
Personal support N/A Personal consultations and support regarding your analysis and results.
Turbo delivery Available 3 weeks (extra charge) Available 2 weeks (extra charge)
Extended data report Available (extra charge) Included :
Extended report contains:
A graphical presentation of
(a) averaged lipid class values with replicate standard deviations,
(b) averaged (sub)species, double bond, chain length and hydroxylation profiles for each lipid class,
(c) principal component analysis for un-averaged replicates.
The extended report can be viewed via internet browser (as an offline local webpage) or data files can be browsed manually.
Interactive data visualization and analysis Available (free of charge with the extended data report)
Interactive LipotypeZoom displays the content of the extended report and is internet-accessible via browser.
Available (free of charge with the extended data report)
Interactive LipotypeZoom displays the content of the extended report and is internet-accessible via browser.




  Standard Analysis Premium Analysis
Delivery time 6 weeks 4 weeks
Minimum 15 samples 20 samples
Structural Details Glycerophospholipids and glycerolipids (except TAG and CL) are reported as subspecies – e.g. PC-36:1;0(18:0;0-18:1;0) denotes PC with the total length of its fatty acids of 36, 1 double bond and 0 hydroxylations that is made up from two fatty acids: 18:0 (stearic) and 18:1 (oleic). The remaining lipid classes are reported as species e.g. – CER-34:1;2. Glycerophospholipids and glycerolipids (except TAG and CL) are reported as subspecies – e.g. PC-36:1;0(18:0;0-18:1;0) denotes PC with the total length of its fatty acids of 36, 1 double bond and 0 hydroxylations that is made up from two fatty acids: 18:0 (stearic) and 18:1 (oleic). The remaining lipid classes are reported as species e.g. – CER-34:1;2.
Approach LipotypeXplorer identifies all measurable lipids in a sample using molecular fragmentation query language without the need to rely on predefined databases. LipotypeXplorer identifies all measurable lipids in a sample using molecular fragmentation query language without the need to rely on predefined databases.
Results Quantitative
Results are expressed in absolute values - pmol per sample volume.
Quantitative
Results are expressed in absolute values - pmol per sample volume.
Standard report contains:
Information on methodology, analysis details, used sample amounts, technical variation and quantitative lipidomics results in tabular format.
contains:
Information on methodology, analysis details, used sample amounts, technical variation and quantitative lipidomics results in tabular format.
Basic coverage PC - phosphatidylcholine
PE - phosphatidylethanolamine
PI - phosphatidylinositol
DAG - diacylglycerol
TAG - triacylglycerol
SM - sphingomyelin
CHOL - free cholesterol
CE - cholesteryl ester
CER - ceramide
LPC - lyso-phosphatidylcholine
LPE - lyso-phosphatidylethanolamine
PC O- - ether-linked PC
PE O- - ether-linked PE
PC - phosphatidylcholine
PE - phosphatidylethanolamine
PI - phosphatidylinositol
DAG - diacylglycerol
TAG - triacylglycerol
SM - sphingomyelin
CHOL - free cholesterol
CE - cholesteryl ester
CER - ceramide
LPC - lyso-phosphatidylcholine
LPE - lyso-phosphatidylethanolamine
PC O- - ether-linked PC
PE O- - ether-linked PE
Extended coverage N/A PA - phosphatidate
PG - phosphatidylglycerol
PS - phosphatidylserine
LPA - lyso-phosphatidate
LPI - lyso-phosphatidylinositol
LPS - lyso-phosphatidylserine
LPC O- - ether-linked LPC
LPE O- - ether-linked LPE
HEXCER - hexosyl ceramide
Personal support N/A Personal consultations and support regarding your analysis and results.
Turbo delivery Available 3 weeks (extra charge) Available 2 weeks (extra charge)
Extended data report Available (extra charge) Included :
Extended report contains:
A graphical presentation of
(a) averaged lipid class values with replicate standard deviations,
(b) averaged (sub)species, double bond, chain length and hydroxylation profiles for each lipid class,
(c) principal component analysis for un-averaged replicates.
The extended report can be viewed via internet browser (as an offline local webpage) or data files can be browsed manually.
Interactive data visualization and analysis Available (free of charge with the extended data report)
Interactive LipotypeZoom displays the content of the extended report and is internet-accessible via browser.
Available (free of charge with the extended data report)
Interactive LipotypeZoom displays the content of the extended report and is internet-accessible via browser.















































  Molecular Metabolism, 2019 
Comprehensive and quantitative analysis of white and brown adipose tissue by shotgun lipidomics
Michal Grzybek, Alessandra Palladini, Vasileia I Alexaki, Michal A Surma, Kai Simons, Triantafyllos Chavakis, Christian Klose, Ünal Coskun
Read abstract : doi.org/10.1016/j.molmet.2019.01.009
  BBA Biomembranes, 2019 
Membrane cholesterol depletion reduces downstream signaling activity of the adenosine A2a receptor
Claire McGraw, Lewen Yang, Ilya Levental, Edward Lyman, Anne Skaja-Robinson
Read abstract : doi.org/10.1016/j.bbamem.2019.01.001
  Nature Communications Biology, 2018 
Dissection of metabolic reprogramming in polycystic kidney disease reveals coordinated rewiring of bioenergetic pathways
Christine Podrini, Isaline Rowe, Roberto Pagliarini, Ana S H Costa, Marco Chiaravalli, Ivano Di Meo, Hyunho Kim, Gianfranco Distefano, Valeria Tiranti, Feng Qian, Diego di Bernardo, Christian Frezza, Alessandra Boletta
Read abstract : doi.org/10.1038/s42003-018-0200
  Patent, 2018  
Compositions for facilitating membrane fusion and uses thereof
Geoffrey A Von Maltzahn, John M Milwid, Michael T Mee, Jacob Rosenblum-Rubens, Nathan Wilson-Stebbins, Molly K Gibson, Neal F Gordon, Bo Zhang, Kyle M Trudeau, Brigham J Hartley, Tamar R Putiri, Kiana Mahdaviani, Matthew Milnes-Dobbin
Read abstract : Downloadfreepatentsonline.com/WO2018208728A1.html
  Science, 2018 
Viscous control of cellular respiration by membrane lipid composition
Itay Budin, Tristan de Rond, Yan Chen, Leanne J G Chan, Christopher J Petzold, Jay D Keasling
Read abstract : doi.org/10.1126/science.aat7925
  White Paper Series, 2018 
Unlocking the Power of Multiomics
Henri Deda, Christian Klose, Kai Simons
Read abstract : Downloadlipotype.com/multiomics/
  Frontiers in Psychiatry, 2018 
Lipidomics in Major Depressive Disorder
Andreas Walther, Carlo V Cannistraci, Kai Simons, Claudio Durán, Mathias J Gerl, Susanne Wehrli, Clemens Kirschbaum
Read abstract : doi.org/10.3389/fpsyt.2018.00459
  Scientific Reports, 2018 
Cholesterol is Inefficiently Converted to Cholesteryl Esters in the Blood of Cardiovascular Disease Patients
Mathias J Gerl, Winchil L C Vaz, Neuza Domingues, Christian Klose, Michal A Surma, Júlio L Sampaio, Manuel S Almeida, Gustavo Rodrigues, Pedro Araújo-Gonçalves, Jorge Ferreira, Claudia Borbinha, João P Marto, Miguel Viana-Baptista, Kai Simons, Otilia V Vieira
Read abstract : doi.org/10.1038/s41598-018-33116-4
  Product development, 2018 
Photodamaged dry facial skin from different ethnic groups
Rainer Voegeli, Jean-Marc Monneuse, Christian Klose, Rotraut Schoop, Beverley Summers, Thomas Rudolph, Anthony V Rawlings
Read abstract : Downloadlipotype.com/2018/08/royal-dsm/
  Journal of Biological Chemistry, 2018 
An oxanthroquinone derivative that disrupts RAS plasma membrane localization inhibits cancer cell growth
Lingxiao Tan, Kwang-Jin Cho, Pratik Neupane, Robert J Capon, John F Hancock
Read abstract : doi.org/10.1074/jbc.RA118.003907
  NPJ Vaccines, 2018 
Activation of the endoplasmic reticulum stress sensor IRE1α by the vaccine adjuvant AS03 contributes to its immunostimulatory properties
Charlotte Givord, Iain Welsby, Sophie Detienne, Séverine Thomas, Assiya Assabban, Viviana Lima Silva, Céline Molle, Romain Gineste, Marjorie Vermeersch, David Perez-Morga, Oberdan Leo, Catherine Collignon, Arnaud M Didierlaurent, Stanislas Goriely
Read abstract : doi.org/10.1038/s41541-018-0058-4
  Patent, 2018 
Methods and systems for metabolite and/or lipid-based detection of colorectal cancer and/or adenomatous polyps
Marko Bitenc, Kristi Kruusmaa, Paola Hurtado-Castillo, Ana M Jiménez-Girón, Rosa Argamasilla-Martinez, Andreu Fabregat-Rossel, Antonio J Adsuar-Gomez, Juan Martinez-Barea, Christian Hense, Patricia Rodríguez-Gómez, Ángela Peralbo-Molina, Jorge Casado-Agrelo, Alejandro Sánchez-Brotons, Christina Pavón-Solís, Rosa M Delgado-Sánchez
Read abstract : Downloadfreepatentsonline.com/y2017/0343567.html
  Neuron, 2018 
ER Lipid Defects in Neuropeptidergic Neurons Impair Sleep Patterns in Parkinson’s Disease
Jorge S Valadas, Giovanni Esposito, Dirk Vandekerkhove, Katarzyna Miskiewicz, Liesbeth Deaulmerie, Susanna Raitano, Philip Seibler, Christine Klein, Patrik Verstreken
Read abstract : doi.org/10.1016/j.neuron.2018.05.022
  Biochemical Journal, 2018  
A phosphatidic acid binding protein is important for lipid homeostasis and adaption to anaerobic biofilm conditions in Pseudomonas aeruginosa
Maike K Groenewold, Marco Massmig, Stefanie Hebecker, Linna Danne, Zofia Magnowska, Manfred Nimtz, Franz Narberhaus, Dieter Jahn, Dirk W Heinz, Lothar Jänsch, Jürgen Moser Read abstract : doi.org/10.1042/BCJ20180257
  Product development, 2018 
Lipid analysis for skin penetration properties of saturated phospholipids
Dorothea Gutekunst, Christian Klose, Christoph Heidecke, Peter Van Hoogevest
Read abstract : Downloadlipotype.com/2018/05/lipoid/
  Phytochemical Analysis, 2018 
Dereplication of plant phenolics using a mass‐spectrometry database independent method
Ricardo M Borges, Rahil Taujale, Juliana Santana de Souza, Thaís de Andrade Bezerra, Eder Lana e Silva, Ronny Herzog, Francesca V Ponce, Jean-Luc Wolfender, Arthur S Edison
Read abstract : doi.org/10.1002/pca.2773
  Proteomics, 2018 
How Can Omic Science be Improved?
Kai Simons
Read abstract : doi.org/10.1002/pmic.201800039
  Cancer Research, 2018 
Organelle-derived acetyl-CoA promotes prostate cancer cell survival, migration, and metastasis via activation of calmodulin kinase II
Guoyu Yu, Chien-Jui Cheng, Song-Chang Lin, Yu-Chen Lee, Daniel E Frigo, Li-Yuan Yu-Lee, Gary E Gallick, Mark A Titus, Leta K Nutt, Sue-Hwa Lin
Read abstract : doi.org/10.1158/0008-5472.CAN-17-2392