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Combinatorial DNA Library Assembly Á¦ÀÛ ¼­ºñ½º

High-throughput DNA library¸¦ Á¦ÀÛÇØµå¸³´Ï´Ù.

Genscript
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  • Custom Á¦ÀÛ: pool or individual formats with up to 1x10^8 constructs containing 4 variable slots for 15 kb inserts
  • metabolic pathway, microbial strain engineering process ¿¬±¸¿¡ ¿ëÀÌ
  • ºü¸£°í °æÁ¦ÀûÀΠÁ¦ÀÛ



 1. Pooled Library Assembly
  • Negative clones¿¡ ¹Î°¨ÇÏÁö ¾ÊÀº ½ºÅ©¸®´× Ç÷§Æû
  • 10^8°³ÀÇ ¼­·Î ´Ù¸¥ ±¸Á¶¸¦ È¥ÇÕÇÏ¿© 1.5ml tube¿¡ Á¦°ø
  • Á¦À۱Ⱓ : ¾à 3ÁÖ


 2. Representative Library Assembly
  • PCR °ËÁõµÈ ÃÖ´ë 10,000°³ÀÇ °³º° construct¸¦ Á¦°ø
  • 10^2 - 10^4 throughput screening: ¹«ÀÛÀ§ÀÇ Individual constructs ÇüÅ·ΠÁ¦°ø
  • Á¦À۱Ⱓ : ¾à 4ÁÖ




 3. Arrayed Library Assembly
  • ½ÃÄö½º °ËÁõµÈ ÃÖ´ë 10,000°³ÀÇ °³º° contruct Á¦°ø
  • Testing every single design in your library
  • Á¦À۱Ⱓ : ¾à 3ÁÖ