특징
- 30분 이내에 다양한 샘플로부터 고농도의 bacterial 및fungal DNA를 추출합니다.
- 특별히 조성된 Buffer MB1과 Lysing Matrix E를 이용하여 효과적으로 추출할 수 있습니다.
- 박테리아, 체액 및 토양과 분변 같은 다양한 환경 샘플로부터 DNA를 추출할 수 있습다.
- 고수율, 고품질의 gDNA를 제공하여 qPCR, restriction digestion, 시퀀싱 등의 downstream application을 할 수 있습니다.
- Organic denaturant를 사용하지 않아 안전합니다.
- Lysis 과정이 간단하고 편리합니다.
구성품
|
50 Rections
(Cat. No. 116553050) |
5 Rections
(Cat. No. 116553000) |
| Components |
Package |
Cat. No. |
Package |
Cat. No. |
| Lysing Matrix E |
50
ea |
116914050 |
5
ea |
116914005 |
| Rnase A Solution |
2
mL |
116546051 |
200
μL |
116546001 |
| Buffer MB1 |
45
mL |
116553053 |
4.5
mL |
116553003 |
| Buffer MB2 |
17
mL |
116553054 |
1.7
mL |
116553004 |
| Buffer MB3 |
21
mL x
2 |
116553055 |
4.2
mL |
116553005 |
| Buffer MB4 |
28
mL |
116553056 |
3
mL |
116553006 |
| Buffer MB5 |
20
mL x
2 |
116553057 |
4
mL |
116553007 |
| Buffer MB6 |
10
mL |
116553051 |
1
mL |
116553001 |
| Column MB |
50
ea |
116553052 |
5
ea |
116553002 |
| 1.5 mL Collection Tubes |
50
ea |
116530060 |
5
ea |
116530010 |
사용 방법
데이터
Microbial Culture Samples
The bacterial DNA extracted with SPINeasy® DNA Kit for Microbiome has higher yield as compared to that of
competitor kits.
그림 1. Comparison of extraction of bacterial gDNA from two different bacteria species (E. coli and S. aureus, ~108 cfu each) using SPINeasy® DNA Kit for Microbiome and competitors kits.
Bronchoalveolar Lavage Fluid (BALF)
The DNA extracted with SPINeasy® DNA Kit for Microbiome has high yield and good DNA integrity.
그림 2. DNA was extracted from 1 mL of BALF using SPINeasy® DNA Kit for Microbiome and other competitor kits. The results from the three replicates were consistent.
Milk
Different types of milk contain different amounts of protein and fat. SPINeasy® DNA Kit for Microbiome has
an effective impurity removal technology and is able to extract gDNA from the three types of milk tested. The DNA extracted with SPINeasy® DNA Kit for Microbiome showed high yield and better purity (especially for fresh milk) than that of competitor kits.
그림 3. Three types of milk, i.e., fresh milk, skim milk, whole milk (10 mL each) were spiked with 500 μL of bacterial solution. The results from the three replicates were consistent.
Human whole blood
The DNA extracted with SPINeasy® DNA Kit for Microbiome showed high yield and purity.
그림 4. 200 μL of human whole blood (spiked with 10 μL of bacterial solution) were added directly to Lysing Matrix E, and used for DNA extraction. SPINeasy® DNA Kit for Microbiome gave the highest yield and good DNA integrity as compared to the competitor kits. The results from the three replicates were consistent. DNA marker: 1,5000 bp
Inhibitor-Free DNA Ready for Downstream Applications
The DNA extracted with SPINeasy® DNA Kit for Microbiome could be directly used for downstream applications (such as qPCR), suggesting the effectiveness of the kit in removing any inhibitor.
그림 5. qPCR amplification of gDNA extracted from Sputum and BALF samples using SPINeasy® DNA Kit for Microbiome. 20 ng of DNA template were used in each reaction. Primers: 16S rRNA (197 bp) and Globin (400 bp)