초고해상도 현미경 관찰에 이용되는 Janelia Fluor® Dyes | 고마바이오텍

특징 

• 자발적으로 깜박이는 Janelia Fluor® Dyes는 광활성화 용액이나 산화환원 완충액 필요없이, 세포와 고밀도 생물분자 구조에서 single-molecule localization microscopy (SMLM)과 같은 초고해상도 현미경 관찰에 사용됩니다. 
• 초고해상도 현미경 실험에 이상적인 duty cycle로 "꺼짐"과 "켜짐" 상태 사이를 자동으로 순환합니다. 

종류 

실험 결과

Application of Janelia Fluor® 635b, Maleimide. 

3D SMLM map of a FUS coacervate aged for 12 h containing 20 nM FUSA2C-JF635b; inset shows brightfield image; scale bar = 4 μm; n = 31,123 total localizations; colors indicate localization density within a 100 nm radius sphere: black = 1; orange 2–10; green = 11–20; cyan = 21–40; magenta = 41–100. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149 

Application of Janelia Fluor® 635b, NHS ester. 

Representative 3D ATAC–SMLM rendering of accessible chromatin localizations in wild-type mouse embryonic stem (mES) cells using oligonucleotide probes labeled with Janelia Fluor® 635b, NHS ester; the color-coded localization density was calculated with a canopy radius of 250 nm; scale bars: 2 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149 

Application of Janelia Fluor® 646b, NHS ester. 

SOFI RNA-FISH image of mouse embryo fibroblast cells expressing MS2 in the 3′ UTR of the beta-actin gene and labeled with Janelia Fluor®646b-oligonucleotide from Janelia Fluor® 646b, NHS ester; scale bar: 5 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149