제품 상세 AXOL 심실심근세포 | Ventricular Cardiomyocyte | Cardiac cells iPSC 유래 human Ventricular Cardiomyocytes는 심장독성 테스트, 약물검사, 약물 유효성 확인 및 신진대사 연구, electrophysiology에 사용되는 심실심근세포입니다. 프린트 제품 문의 특징 iPSC-derived human ventricular cardiomyocyte는 심부전, 심장독성 테스트, 약물 유효성 확인, 신진대사 연구, 전기생리학 실험 등에 사용됩니다.해동 후 3일 안에 in vitro에서 자발적으로 박동하기 시작하며, 7일안에 assay에 사용할 수 있습니다.Troponin을 포함한 심장 특이 마커를 발현합니다. 28가지 CiPA compound에 validation되었으며, 약물 평가 및 cardiotoxicity 스크리닝에 사용됩니다. 사양 Starting material Fibroblasts Donor gender Male Donor age at sampling 74 yrs Reprogramming method Episomal vector Induction method Fully defined medium Size 1 million cells Growth properties Adherent Disease information Healthy 적용 Electrophysiology : Contractility, QT prolongation, Na+ & Ca2+ channels, Pharmacology 실험Functional Integrity : Ca2+ signaling, Morphology, Stress & toxic response markers 확인 제품 Catalog. No. Product Name Format Conc. ax2508 Human iPSC-Derived Ventricular Cardiomyocytes (Male) 1.0 million cells/vial ax2518 Human iPSC-Derived Atrial Cardiomyocytes (Male) 1.0 million cells/vial ax2530-500 Cardiomyocyte Maintenance Medium 1 x 500 mL Basal Medium 1 x 10 mL Supplement ax0050 Fibronectin Coating Solution 1 mL ax2500 Human iPSC-Derived Ventricular Cardiomyocyte Kit Cell (ax2508) + Media (ax2530-500) + Fibronectin (ax0050) 참고 데이터1. Gene expression : Pan-cardiac, atrial, ventricular marker genes의 발현 비교2. Immunohistochemistry : Cardiomyocyte 특이적인 marker를 발현합니다.Axol Human iPSC-Derived Ventricular Cardiomyocytes were cultured in Cardiomyocyte Maintenance Medium prior to being fixed and stained for the markers Sarcomeric Alpha Actinin and Cardiac Troponin. The cells were counterstained using DAPI to allow visualization of nuclei.Expression of Troponin T, atrial natriuretic peptide (ANP) and atrial myosin light chain 2 (MLC2a) was confirmed in Axol iPSC-Derived Atrial Cardiomyocytes using immunocytochemistry. Troponin T staining (red) confirmed the presence of cardiac myocytes which are responsible for sarcomere contraction. ANP is specifically secreted by atrial myocytes upon atrial stretching and MLC2a facilitates cardiac contractility wo. DAPI counterstain. 3. Beating : 배양 후 자발적으로 박동합니다.4. Action potential characteristics : patch clamp에서 자발적인 action potential을 보여줍니다.Characteristics of spontaneous and evoked action potentials recorded from Axol’s Human iPSC-derived Ventricular Cardiomyocytes. Spontaneous (A) and evoked (1 Hz; B) action potentials recorded by whole-cell patch clamp. (C) Average action potential parameters for spontaneous and evoked action potentials in control conditions.5. Core cardiac ion channel pharmacology : INa, ICa,L and IKr을 발현합니다.Axol’s Human iPSC-derived Ventricular Cardiomyocytes express the core cardiac ion channels INa, ICa,L and IKr. (A) Representative traces of evoked action potentials recorded under control conditions (grey) and in the presence of 100 μM Lidocaine (green), 100 nM Nifedipine (blue) or 50 nM Dofetilide (red), which show expected effects on action potential amplitude and duration. B) Average effect of each compound on spontaneous action potential parameters, presented as percent of control ± SEM, N ≥ 4. Statistical significance calculated by a paired two-tailed Student’s t-test (* p<0.05, ** p<0.01, *** p<0.001).6. Modeling Arrhythmias : 부정맥 분석에 사용할 수 있습니다.Axol’s Human iPSC-Derived Ventricular Cardiomyocytes were treated with doxorubicin (control, 500 nM, 100 nM and 50 nM). There was a dose-dependent response at 13 h and 24 h post-treatment with doxorubicin. Arrhythmias and loss of beating amplitude were seen at lower doxorubicin concentration, whereas higher doxorubicin concentrations resulted in tachycardia and ultimately cell death. Data kindly provided by Dr J. Maynes, University of Toronto.7. CiPA(Comprehensive in vitro Pro-Arrhythmia Assay) : 약물의 심장독성 확인에 이용됩니다.Na+와 K+를 block하는 Quinidine을 테스트. Axol의 Cardiomyocyte는 낮은 농도에서 triangulation의 증가를 확인Anti Dopaminergic Drug인 Droperidol을 처리하여, 가장 낮은 농도에서 QT prolongation을 확인관련 자료How To Videos and GuidesHow to seed atrial cardiomyocytes on Multi-Electrode Array (MEA)How to use Microelectrode Array (MEA) to assess the electrophysiological properties of iPSC-derived Atrial and Ventricular CardiomyocytesPostersIn vitro validation of human iPSC-derived atrial cardiomyocytes for cell-based assays and drug discoveryFunctional characterisation of human iPSC-derived atrial cardiomyocytesAre stem-cell cardiomyocytes a viable cellular reagent for automated patch-clamp?In vitro assessment of excitation-contraction coupling for predicting pro-arrhythmic risk in iPSC-derived ventricular cardiomyocytesIn vitro assays and screening platforms for exploring ventricular and atrial phenotypes in iPSC-derived cardiomyocytesSerum-free human iPSC-derived cardiomyocytes for in vitro testingEffects of transient hypoxia/ischemia on inducible human pluripotent stem cell (iPSC)-derived cardiomyocytesA drug discovery platform for the identification of novel infarct sparing agents for treatment of ischemic heart diseaseProduct citationsMajidi S.S, Slemming-Adamsen P, Hanif M et al., Wet electrospun alginate/gelatin hydrogel nanofibers for 3D cell culture. International Journal of Biological Macromolecules (2018)Shen Y, Dana H, Abdelfattah A.S, et al., A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein. Biorxiv (2018)Sutton K, Ridly J, El Haou S, et al., Human Stem Cell-Derived Cardiomyocytes: In Vitro Assays and Screening Platforms for Exploring Ventricular and Atrial Phenotypes. Journal of Pharmacological and Toxicological Methods (2017)Martella D, Paoli P, Pioner J.M, et al., Liquid Crystalline Networks toward Regenerative Medicine and Tissue Repair. Small - Wiley Online Library (2017)Papp R, Bett GCL, Lis A, et al., Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women. BioMed Central (2017)Zuppinger C, Gibbons G, Dutta-Passecker P, et al., Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. European Journal of Histochemistry (2017)Chang Y, Broyles CN, Brook FA, et al., Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics. PLoS One (2017)White PaperImproving biological tools for disease modelling and drug discovery: human iPSC-derived Atrial CardiomyocytesInduced Pluripotent Stem Cells: recapitulating disease to facilitate drug discoveryBlogsThree reasons why iPSC-derived atrial cardiomyocytes are poised to accelerate atrial fibrillation researchiPSC-derived atrial cardiomyocytes: a reproducible platform for translational disease insightsiPSC-derived male and female cardiomyocytes for cardiovascular researchHuman iPSC-derived cardiomyocytes: studying cardiomyocytes in vitroMicroRNA and the Heart 주문정보 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS Human iPSC-Derived Ventricular Cardiomyocyte Kit (male) ax2500 1 kit AXOL - + 보기 보기 Human iPSC-Derived Ventricular Cardiomyocytes (Male) ax2508 1 kit AXOL - + 보기 보기 Cardiomyocyte Maintenance Medium ax2530-500 500 ml AXOL - + 보기 보기 Fibronectin Coating Solution ax0050 (1mL) 1 ml AXOL - + 보기 보기 Normal Human Cardiac Fibroblasts ax3039 1 vial AXOL - + 보기 보기 1 Maker AXOL Cat.No. ax2500 Product Human iPSC-Derived Ventricular Cardiomyocyte Kit (male) Size 1 kit Qty - + Data Sheet 보기 2 Maker AXOL Cat.No. ax2508 Product Human iPSC-Derived Ventricular Cardiomyocytes (Male) Size 1 kit Qty - + Data Sheet 보기 3 Maker AXOL Cat.No. ax2530-500 Product Cardiomyocyte Maintenance Medium Size 500 ml Qty - + Data Sheet 보기 4 Maker AXOL Cat.No. ax0050 (1mL) Product Fibronectin Coating Solution Size 1 ml Qty - + 5 Maker AXOL Cat.No. ax3039 Product Normal Human Cardiac Fibroblasts Size 1 vial Qty - + first 1 last 추천제품 Cardiovascular System Small Molecules 심장독성 평가 (hERG Assay & CiPA) | Cardiac Safety 서비스 Cardiac Marker ELISA Kit 이온 채널 Inhibitor | Ion Channel Agonist and Antagonist AXOL 심방심근세포 | Atrial Cardiomyocyte Kit 관련제품 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS 자료 Cells 세포실험 제품가이드 Vol.2 [고마바이오텍] 면역세포, 신경세포 등 다양한 세포 및 세포 실험에 사용하는 제품을 소개합니다. PDF 웨비나/Video
Cardiovascular System Small Molecules 심장독성 평가 (hERG Assay & CiPA) | Cardiac Safety 서비스 Cardiac Marker ELISA Kit 이온 채널 Inhibitor | Ion Channel Agonist and Antagonist AXOL 심방심근세포 | Atrial Cardiomyocyte Kit