PEI STAR™ DNA Transfection Reagent | 고마바이오텍

특징

• PEI STAR는 일시적인 유전자 발현을 위한 polyethylenimine (PEI) transfection regent 입니다.
  
• PEI는 중성 pH에서 높은 양전하를 띠는 합성 폴리머로, 음전하를 띠는 DNA에 강하게 결합하여 세포내로 들어갈 수 있도록 합니다. 
• HEK293, CHO cell 및 기타 다양한 진핵세포 transfection에 이용됩니다. 
• PEI STAR-GO (#8174)는 바로 사용할 수 있는 용액 형태의 PEI STAR (#7854) 입니다.
• Application : lentivirus, rAAV, recombinant proteins, antibody 생산 

사용법    

참고 데이터

PEI STAR™ performance data comparison (HEK293) 

HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance. 

PEI STAR™ performance data comparison (CHO) 

CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance. 

Transfection of a reporter GFP construct using a leading competitor PEI and PEI STAR™. 

HEK293 cells grown in DMEM/10% FBS to 75% confluency in a 24 well plate. 1 μg of DNA + 25 μL optimem and 2 μg of PEI (1 mg/mL) + 25 μL optimem were incubated for 8 minutes before addition to cells. PEI STAR™ showed significantly higher expression of GFP in imaged cells.